Molecular evolution of hepatitis A virus in a human diploid cell line

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Sep 14, 2007 (publication date) through Apr 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 14, 2007; 13(34): 4630-4635
Published online Sep 14, 2007. doi: 10.3748/wjg.v13.i34.4630

Table 1 Primers used in amplifying HAV genome

Name	Sequences	Position
F1	T7¹-TTTCCGGAGYCCCTCTTG	22-39
R1	M13R²-ATCTGCCAGAGACAGGA	806-790
F2	T7-GAGGTACTCAGGGGCATTTA	690-709
R2	M13R-GGATCCAAAGCAAAAGACA	1567-1549
F3	T7-AGATTTGGAGTTGCATGGATTAAC	1430-1453
R3	M13R-GGATCCTGAAACATCCA	2342-2326
F4	M13R-TTCAACAACAGTTTCTACAG	2231-2250
R4	T7-AGCTTCCTCCTCTGATCTA	3098-3080
F5	T7-GGGAGATAAGACAGATTCTACAT	2861-2883
R5	M13R-AGCTTCCACTCTTAACAAA	3813-3795
F6	M13R-GAATGCTTGGATTGTCTGG	3634-3652
R6	T7-GATCCATCCCAGTAATCTGA	4549-4530
F7	T7-ATCAATTGCATTGGCAAC	4451-4468
R7	M13R-GGATCCGCATCTAATTTAATC	5278-5258
F8	M13R-GCTGTGGGAGCTGCAGTTGG	5124-5143
R8	T7-GATCCATGGGACTCTTTCTA	6027-6008
F9	T7-GAGGAAATTCAATTCTTG	5872-5889
R9	M13R-GGATGGAGTTCCAGA	6764-6750
F10	T7-GGAGATGTYGGTCTTGAT	6657-6674
R10	M13R-AGAGGGGTCTCCGGGAATTT	polyA
3'RACE	CAAGAGGGGTCTCCGGGAATTTTTTTTTTTTTTTTTTTT	polyA

¹T7 indicates adapter with sequence: TAATACGACTCACTATAGGGAGA,

²M13R indicates adapter with sequence: AGCGGATAACAATTTCACACAGGA.

Table 2 Divergence of H2w and its progenies compared to reference strains in VP1/2A region (%)

Citation: Tang CH, Mao JS, Chai SA, Chen Y, Zhuang FC. Molecular evolution of hepatitis A virus in a human diploid cell line. World J Gastroenterol 2007; 13(34): 4630-4635
URL: https://www.wjgnet.com/1007-9327/full/v13/i34/4630.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i34.4630