RhoA and RhoC -siRNA inhibit the proliferation and invasiveness activity of human gastric carcinoma by Rho/PI3K/Akt pathway

Table 1 The invasion cell number of human SGC7901 cell after treatment of Ad- RhoA and RhoC-siRNA (%) (mean ± SEM)

	RhoA & C-siRNA	RhoA -siRNA	RhoC -siRNA	siRNA	LY294002
Invading cells	12.64 ± 3.27	38.26 ± 14.75	35.27 ± 10.94	87.38 ± 17.38	37.01 ± 12.38

Table 2 The apoptosis rate of 5 groups with FACS (%) (mean ± SEM)

Group	Apoptosis rate (%)	P
1 RhoA,C-siRNA	19.07 ± 1.78	Compared with any group (P < 0.01 or P < 0.05)
2 RhoA-siRNA	7.13 ± 0.21	Compared with 4 or 5 group (P < 0.05), compared with 3 group (P > 0.05)
3 RhoC-siRNA	8.11 ± 0.17	Compared with 4 or 5 group (P < 0.05)
4 siRNA	1.23 ± 0.11	Compared with any group (P < 0.01 or P < 0.05)
5 LY294002	9.44 ± 1.28	Compared with 4 group (P < 0.01)

Table 3 The experiment of transplantation tumor in BALB/C nude mice (mean ± SEM)

Group	Volume after 2 wk (mm3)	t	P	Weight after 2 wk (g)	t	P	Rate of inhibition (%)
							Volume	Weight
RhoA & C-siRNA	37.7 ± 5.65	7.216	0.001	0.245 ± 0.116	4.121	0.013	51.7	56.8
RhoA-siRNA	67.7 ± 2.12	4.776	0.003	0.311 ± 0.123	3.011	0.013	27.2	21.3
RhoC-siRNA	65.7 ± 5.78	4.346	0.003	0.323 ± 0.121	3.012	0.013	25.7	25.8
siRNA	97.7 ± 3.33			0.555 ± 0.132		0.013	7.1	2.3
LY294002	49.7 ± 4.21	6.116	0.003	0.297 ± 0.161	3.453	0.013	29.3	23.4

P < 0.05 vs siRNA.