Alteration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation

Table 1 Proteins identified by MALDI-TOF-MS

Spot No.	NCBInr entry	Protein name	Mw/pI	Sequencecoverage(%)	Biological function
Up-regulated
No. 12	gi13785574	p27BBP protein/eIF6	26 845/4.56	27	β4 integrin interactor, a component of NM filaments escorts 60S ribosome subunit from nuclear to cytoplasm
No. 16	gi88983724	Similar to TFIIH	36 539/6.79	23	Basic transcriptin factor 2 44 kDa subunit (BTF2-p44) (General transcription factor)
		Basal transcription factor complex p44 subunit
No. 18	gi14043898	Tubulin, beta 2C	50 255/4.79	17	Tubulin β subunit, with GTPase activity
	gi27368062	Class IVb beta tubulin	50 177/4.82	17
No. 21	gi13477279	SIN3B protein	49 609/6.56	23	Interact with the Mad components of the Myc/Max/Mad network of cell growth regulators
New protein
No. 14	gi45708738	SFRS1 protein	22560/7.72	25	arginine/serine-rich 1 alternate splicing factor
Down-regulated
No. 5	gi5822131	Crystal structure of an active site mutant of the pyst1	16364/5.31	47	ERK-specific MAP kinase phosphatase PYST1/MKP3
No. 7	gi62088338	Hypothetical protein: DKFZp434K1815 variant	26 864/5.64	24	From Deutches Krebs-Forschungszentrum cancer research centre (German)
No. 19	gi34234	Laminin-binding protein	31 888/4.84	28	Laminin-binding protein, LBP
	gi250127	67 kDa laminin receptor	32 860/4.83	27
	gi17939541	Integrin beta 4 binding protein	27 095/4.56	26
No. 20	gi83641870	Nucleophosmin1	28 497/4.56	23	Also known as NPM, numatrin, No38, B23 protein
		Isoform 3
	gi40353734	Nucleophosmin 1	29 617/4.47	23	A major nucleolus phosphoprotein, a component of NM
		Isoform 2