Expression of p21WAF1 is related to acetylation of histone H3 in total chromatin in human colorectal cancer

Table 1 Primer sequence, PCR program and Genbank accession number

Primers	Sense (5’ -> 3’)		Antisense (5’ -> 3’)		Size and PCR condition	GenBank accession number
β-actin	GGC	ATC	GCT	GGA	94°C 5 min; 92°C 40 s;	BC023204
	GTG	ATG	AGG	TGG	58°C 40 s; 72°C 50 s, 30X
	GAC TCC G		ACA GCG A
p21WAF1	CAG	GGG	GGG	CGG	94°C 5 min; 94°C 1 min;	NM_000389
	ACA	GCA	CCA	GGG	58°C 1 min; 72°C 1 min, 35X
	GAG GAA GA		TAT GTA C

Table 2 Sequences of primers and probes for real-time PCR

Gene	Primer (forward) (5’ -> 3’)	Primer (reverse) (5’ -> 3’)	Probe	GenBank No.
p21WAF1	CTG GAG ACT	GGA TTA GGG	ACG GCG GCA	NM_078467
	CTC AGG GTC	CTT CCT CTT	GAC CAG CAT GA
	GAA	GGA
β-actin	CTG GCA CCC	GGA CAG CGA	ATC ATT GCT	BC016045
	AGC ACA ATG	GGC CAG GAT	CCT CCT GAG

Table 3 Expression of p21^WAF1 mRNA in colorectal carcinoma tissue

	Canceroustissues	Para-cancerous	Normal mucosa
p21WAF1 mRNA	2377.95 ± 865.80b	3216.58 ± 1149.42	3541.61 ± 433.17

^bP < 0.01: average density in cancerous tissue vs that in corresponding para-cancerous tissue and normal colorectal mucosa. The band density detected by RT-PCR was described in the “Material and Methods”.