Zahn A, Moehle C, Langmann T, Ehehalt R, Autschbach F, Stremmel W, Schmitz G. Aquaporin-8 expression is reduced in ileum and induced in colon of patients with ulcerative colitis. World J Gastroenterol 2007; 13(11): 1687-1695 [PMID: 17461471 DOI: 10.3748/wjg.v13.i11.1687]
Corresponding Author of This Article
Alexandra Zahn, MD, Department of Gastroenterology, University Hospital of Heidelberg, Im Neuenheimer Feld 410, D-69120 Heidelberg, Germany. alexandra.zahn@med.uni-heidelberg.de
Article-Type of This Article
Clinical Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Mar 21, 2007; 13(11): 1687-1695 Published online Mar 21, 2007. doi: 10.3748/wjg.v13.i11.1687
Table 1 Patients’ characteristics
Disease
Controls
CD
UC
n
4
4
4
Sex (M/F)
1/3
1/3
2/2
Age, range (yr)
52-60
23-43
20-48
Age, mean (yr)
56
32
35
Table 2 Sequences of primers used for PCR amplification of AQP8 promoter and coding region
AQP8 fragment
Primer designation
5’-3’ sequence
Promotor region
Pro-forward
CCGTGTTAGCCAGGATGG
Pro-reverse
GAAACCTGCACCTGCTGTG
Exon 1
Ex1-forward
CCCTGCCCTGTTGAGATTTA
Ex1-reverse
AGGGAAAAAGGGACAGAGGA
Exon 2
Ex2-forward
AAGAGTCCGATGTTTGTGCC
Ex2-reverse
CTTTGCTTTCCACACCCAGT
Exon 3
Ex3-forward
ACACTGTCTCAAGTGCCAGC
Ex3-reverse
CACACCCACATATGCACCTC
Exon 4
Ex4-forward
GCAGGGTCGCACAGTAAAAT
Ex4-reverse
CTGGCCCCTAATAGCAACTG
Exon 5
Ex5-forward
AGCCCCTCTGCCTTCTTTAG
Ex5-reverse
TTCCAAACCCAAGTGAGAGC
Exon 6
Ex6-forward
AGCCTGGAGACATGACGAAG
Ex6-reverse
AGAGCCTCCTCAGCAGTCAG
Table 3 Sequences of primer and probes used for TaqMan allelic discrimination of AQP8 SNPs
AQP8 SNP
Primer designation
5’-3’ Sequence
AQP8-A212T
Forward primer
CTTGGTGCCTGGGTGTTTG
Reverse primer
AGCCAGTAGATCCAGTGGAAGTTC
FAM-Probe
CATGAATCCCACCCGT
VIC-Probe
TCCCGCCCGTGCT
AQP8-A260P
Forward primer
GAGATGGGAAGACCCGCC
Reverse primer
GGAATCCCACGAGCTCTGC
FAM-Probe
TCCTGAAGGCTCGGTGA
VIC-Probe
TCCTGAAGCCTCGGTGA
AQP8-R261Q
Forward primer
GGCAGGAAATGCAGGAACTC
Reverse primer
GGGAAGACCCGCCTCATC
FAM-Probe
AGCTCTGCTTCACTGAG
VIC-Probe
AGTTCTGCTTCACCGAG
Table 4 Selection of differentially expressed genes located in IBD candidate loci
IBDcandidatelocus/Gene
Controlexpressionileum
FCCD
FCUC
Controlexpressioncolon
FCCD
FCUC
Chr. 16q11-12
MMP2
267
1.5
-1.1
220
1.5
3.7
TM4SF11
194
1.3
1.0
151
-2.1
-1.7
SIAH1
208
1.6
1.2
156
3.5
1.3
MT1G
3053
-1.1
1.2
2356
-1.7
-2.0
NETO2
49
-1.6
-1.7
61
2.5
1.4
Chr. 12q13
DGKA
427
-1.5
1.0
342
-2.3
-2.6
MYO1A
1008
1.1
1.1
811
-3.5
-4.3
Chr. 5q32
SPARC
76
2.6
1.5
104
2.0
2.6
TGFBI
236
2.0
1.0
356
1.5
2.5
CAMLG
93
2.5
1.4
91
2.1
1.4
TCF7
357
-2.0
-1.2
279
-1.2
-1.6
SLC35A4
535
-1.5
-1.5
638
-2.3
-1.5
SEC24A
221
2.3
1.1
211
1.7
1.1
SLC26A2
302
2.3
-2.5
388
6.5
-7.5
EGR1
256
2.3
1.1
180
17.1
3.0
PACAP
150
-2.8
-1.7
138
-1.6
2.0
Chr. 19p13
AES
222
-2.1
-1,1
257
-2.5
-2.3
MAP2K2
420
-2.0
-1,1
627
-2.1
-5.7
Chr. 1p36
CDC42
1428
1.1
-1.2
1429
2.0
-1.9
MFN2
304
-1,1
1.0
367
-3.0
-2.5
C1QA
336
1.1
1.1
373
-1.6
-2.5
Chr. 16p12
MIR16
201
-1.7
-1.1
277
-3.0
-1.2
AQP8
575
-8.6
-9.8
438
4.3
3.0
MT1G
3053
-1.1
1.2
2356
-1.7
-2.0
LITAF
500
-1.2
-1.2
670
1.9
2.1
PRKCB1
157
-2.0
1.4
95
1.1
-1.2
USP7
184
1.6
-1.3
192
2.0
-1.1
Chr. 7q11
WBSCR22
172
-1.7
-1.1
207
-1.9
-2.3
POR
213
-2.1
1.0
172
-2.3
-4.9
SEMA3C
79
1.1
1.0
70
2.3
1.9
CLDN3
386
-1.4
-1.2
618
-2.6
-6.5
WBSCR20A
323
-1.6
1.0
281
-2.0
-1.7
TMPIT
928
1.1
1.1
893
-2.3
-2.3
CLDN4
150
1.1
1.0
232
-1.4
-2.3
CD36
153
2.8
1.3
127
2.3
1.1
HSPB1
376
-3.2
-2.3
493
-1.9
-1.5
Chr. 4q25
ELOVL6
112
1.2
-1.6
332
2.1
1.6
ENPEP
578
1.7
1.6
533
1.1
-11.3
T2BP
322
1.2
1.1
224
2.8
1.4
SYNPO2
242
2.3
1.3
160
2.5
1.1
SEC24B
105
2.0
1.6
161
1.6
1.1
Chr. 3p21
ACY1
744
1.1
1.2
485
-2.1
-1.5
Chr. 3q27
AP2M1
417
-1.6
1.0
503
-2.3
-3.5
TNIK
143
2.1
1.1
135
1.4
1.0
PRKCI
68
2.1
1.2
104
2.0
1.1
SI
1313
1.3
1.1
963
1.4
-2.0
EIF4G1
292
-2.5
-1.6
319
-3.7
-6.1
KLHL6
323
-3.2
1.0
110
1.1
1.7
FXR1
137
1.9
1.2
105
2.1
2.0
Chr. 10q22
MYST4
73
2.0
1.4
75
2.0
1.5
RAI17
363
1.6
1.0
250
1.7
2.1
Table 5 Aquaporin gene expression and regulation in IBD
Gene
Controlexpression ileum
FC CD
FC UC
Controlexpression colon
FC CD
FC UC
AQP1
181
1.7
1.7
165
-2.0
-1.6
AQP3
473
1.9
1.5
352
-1.4
-3.2
AQP8
575
-8.6
-9.8
438
4.3
3.0
AQP11
510
1.2
1.0
409
-1.9
-2.0
Table 6 Allelic contribution of AQP8 SNPs
SNP
n VIC
% VIC
n FAM
% FAM
n both
% both
P-Value
χ2
AQP8-A212T
G
G
A
A
G/A
G/A
GCC -> ACC
UC
210
95.5
0
0.0
9
4.1
0.57
0.33
CD
166
91.7
0
0.0
9
5.0
0.98
0.00
Co
232
92.8
0
0.0
14
5.6
AQP8-A260P
G
G
C
C
C/G
C/G
GCT -> CCT
UC
94
42.7
33
15.0
78
35.5
0.26
2.66
CD
56
30.9
26
14.4
83
45.9
0.46
1.55
Co
93
37.2
43
17.2
107
42.8
APQ8-R261Q
G
G
A
A
G/A
G/A
CGG -> CAG
UC
205
93.2
0
0.0
13
5.9
0.62
0.97
CD
159
87.8
1
0.6
9
5.0
0.97
0.07
Co
214
85.6
1
0.4
13
5.2
Citation: Zahn A, Moehle C, Langmann T, Ehehalt R, Autschbach F, Stremmel W, Schmitz G. Aquaporin-8 expression is reduced in ileum and induced in colon of patients with ulcerative colitis. World J Gastroenterol 2007; 13(11): 1687-1695