Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method

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Mar 21, 2007 (publication date) through Apr 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 21, 2007; 13(11): 1672-1679
Published online Mar 21, 2007. doi: 10.3748/wjg.v13.i11.1672

Table 1 Primers used in the present study

Marker	Size	Sense	Antisense	Tm
Pdx-1	451	ACCATGAACAGTGAGGAGCA	TCCTCTTGTTTTCCTCGGGT	55
Foxa2	199	GACAAGGGAAATGAGAGGCTG	ACCAAAGGCTCCTTTAAAACAAG	55
Insulin I	197	CCAGCTATAATCAGAGACCA	GTGTAGAAGAAGCCACGCT	55
Sox 17	247	TCGGACTATGCAGTGTCCGTA	GAAGGTTGCCCGGGGCCGTG	55
Somatostatin	315	ACTAGTCTGCGCTCTGCATCGTCCTG	CCGCGGTCTTCCAGAAGAAGTTCTTG	55
Glucagon	253	ACTAGTGCAGCACGCCCTTCAAGACA	CCGCGGAGCATGTCTCTCAAATTCAT	60
Mixl1	305	GCACGTCGTTCAGCTCGGAGCAGC	AGTCATGCTGGGATCCGGAACGTGG	60
Oct-4	201	TGTGGACCTCAGGTTGGTCT	CTTCTGCAGGGCTTTCATGT	55
GAPDH	179	CATCATCCCTGCCTCTACTG	GCCTGCTTCACCACCTTC	55

Citation: Lin HT, Kao CL, Lee KH, Chang YL, Chiou SH, Tsai FT, Tsai TH, Sheu DC, Ho LL, Ku HH. Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method. World J Gastroenterol 2007; 13(11): 1672-1679
URL: https://www.wjgnet.com/1007-9327/full/v13/i11/1672.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i11.1672