Distinct toll-like receptor expression in monocytes and T cells in chronic HCV infection

Table 1 Patient characteristics

Parameter		Value
Gender (male/female)		12/2
Age (yr)		44 ± 12
Duration of disease	> 10 yr	5
	Unknown	9
Viral load (by QUNT BDNA, IU/ml)		1 x 107 ± 0.78 x 107
Viral genotype (by PCR)	1a	5
	1b	4
	3a	3
	4a	2
Plasma SGOT (ALT) levels (IU/mL)		110 ± 86
Plasma SGPT (AST) levels (IU/mL)		102 ±60

Table 2 Real-time PCR primers

Primer identification		Sequence
TLR1	Forward	5'-GGG TCA GCT GGA CTT CAG AG-3'
	Reverse	5'-AAA ATC CAA ATG CAG GAA CG-3'
TLR2	Forward	5'-GCC TCT CCA AGG AAG AAT CC-3'
	Reverse	5'-TCC TGT TGT TGG ACA GGT CA-3'
TLR3	Forward	5'-GTG CCA GAA ACT TCC CAT GT-3'
	Reverse	5'-TCC AGC TGA ACC TGA GTT CC-3'
TLR4	Forward	5'-AAG CCG AAA GGT GAT TGT TG-3'
	Reverse	5'-CTG AGC AGG GTC TTC TCC AC-3'
TLR5	Forward	5'-TTG CAT CCA GAT GCT TTT CA-3'
	Reverse	5'-TTC AAC TTC CCA AAT GAA GGA-3'
TLR6	Forward	5'-GAA CAT GAT TCT GCC TGG GT-3'
	Reverse	5'-GCT GTT CTG TGG AAT GGG TT-3'
TLR7	Forward	5'-AAT GTC ACA GCC GTC CCT AC-3'
	Reverse	5'-GCG CAT CAA AAG CAT TTA CA-3'
TLR8	Forward	5'-TGT GAT GGT GGT GTC TCA AT-3'
	Reverse	5'-ATG CCC CAG AGG CTA TTT CT-3'
TLR9	Forward	5'-ATT CTG ACT TTG CCC ACC TG-3'
	Reverse	5'-GCT GAG GGA CAG GGA TAT GA-3'
TLR10	Forward	5'-GGC CAG AAA CTG TGG TCA AT-3'
	Reverse	5'-AAA TGA CTG CAT CCA GGG AG-3'
MyD88	Forward	5'-GAG CGT TTC GAT GCC TTC AT-3'
	Reverse	5'-CGG ATC ATC TCC TGC ACA AA-3'
MD2	Forward	5'-ATT CCA AGG AGA GAT TTA AAG CAA TT-3'
	Reverse	5'-CAG ATC CTC GGC AAA TAA CTT CTT-3'
CD14	Forward	5'-CGC TCC GAG ATG CAT GTG-3'
	Reverse	5'-TTG GCT GGC AGT CCT TTA GG-3'