Down-regulation of Bcl-XL by RNA interference suppresses cell growth and induces apoptosis in human esophageal cancer cells

Table 1 Target sequences, corresponding oligonucleotides and resulting siRNA-expressing vectors in this study

Target sequences and corresponding oligonucleotides	siRNA-expressing vector
Target 1: 5´-ggaagagaacaggactgaggc-3´ (Target site: 90-110)	siRNA- expressing vector No.1
Oligo 1 F: 5´-tcgaggaagagaacaggactgaggcttcaagagagcctcagtcctgttctcttccttttt-3´
Oligo 1 R: 5´-ctagaaaaaggaagagaacaggactgaggctctcttgaagcctcagtcctgttctcttcc-3´
Target 2 :5´- gaacaggtagtgaatgaactc-3´(Target site: 370-390)	siRNA- expressing vector No.2
Oligo 2 F: 5´-tcgagaacaggtagtgaatgaactcttcaagagagagttcattcactacctgttcttttt-3´
Oligo 2 R: 5´-ctagaaaaagaacaggtagtgaatgaactctctcttgaagagttcattcactacctgttc-3´
Target 3: 5´- gaacgcttcaaccgctggttc-3´(Target site: 622-642)	siRNA-expressing vector No.3
Oligo3 F: 5´-tcgagaacgcttcaaccgctggttcttcaagagagaaccagcggttgaagcgttcttttt-3´
Oligo 3 R: 5´-ctagaaaaagaacgcttcaaccgctggttctctcttgaagaaccagcggttgaagcgttc-3´
Control: 5´-gaggaccgttactagatcata-3´	Control siRNA- expressing vector
Oligo F: 5´-tcgagaggaccgttactagatcatattcaagagatatgatctagtaacggtcctcttttt-3´
Oligo R: 5´-ctagaaaaagaggaccgttactagatcatatctcttgaatatgatctagtaacggtcctc-3´