Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice

Table 1 Microscopic assessment of histologic changes in DSS colitis

A: Grade of crypt lesion	Score
Intact crypt	0
Grade 1: Loss of the basal third	1
Grade 2: Loss of two thirds	2
Grade 3: Loss of entire crypt	3
Grade 4: Erosion	4
B: Extension of each grade of crypt lesion as % of total mucosal surface	0-100
Crypt lesion score	Σ (A × B)
Grade of inflammation, epithelial regeneration or crypt distortion	Score
Absent	0
Mild	1
Moderate	2
Severe	3

Crypt lesion, acute and chronic inflammation, epithelial regeneration and crypt distortion are scored separately.

Table 2 Histologic scores on d 5 and 12 after Induction of colitis by DSS

	D 5		D 12
	WT	KO	WT	KO
Crypt lesion	40.0 ± 4.0	21.0 ± 5.0a	18.0 ± 2.4	9.5 ± 2.4a
Acute inflammation	1.0 ± 0.2	0.9 ± 0.1	1.8 ± 0.2	0.5 ± 0.15a
Chronic inflammation	0.5 ± 0.1	0.6 ± 0.1	1.5 ± 0.10	0.4 ± 0.1a
Epithelial regeneration	0.2 ± 0.05	0.1 ± 0.02	1.2 ± 0.15	0.95 ± 0.2
Crypt distortion	0	0	0.6 ± 0.10	0.5 ± 0.2

^aP < 0.05 vs WT.