Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues

Table 1 Methylation status of the promoter region of MAGE-A1 and MAGE-A3 genes in 32 colorectal cancer cell lines

	Cell line	MAGEs expression				MS-PCR
		MAGE-A1		MAGE-A3		MAGE-A1		MAGE-A3
		-5-aza/+5-aza		-5-aza/+5-aza		M/U		M/U
1	SNU-61	-	++	±	++	+	+	-	+
2	SNU-81	-	++	-	+++	+	-	+	-
3	SNU-175	++	++	+++	+++	+	+	+	+
4	SNU-283	+++	NT	+++	NT	+	+	+	-
5	SNU-407	+++	+	+++	++	+	-	+	+
6	SNU-503	++	NT	-	NT	+	+	+	+
7	SNU-769A	+++	++	+++	+++	-	+	-	+
8	SNU-769B	+++	+++	+++	+++	-	+	-	+
9	SNU-1033	++	+	++	+++	+	+	+	+
10	SNU-1040	-	-	±	++	+	-	+	-
11	SNU-1047	+++	++	++	++	+	-	-	+
12	SNU-1197	++	+++	++	+++	+	+	+	+
13	SNU-C1	±	NT	++	NT	+	+	-	+
14	SNU-C2A	++	NT	+++	NT	+	+	+	+
15	SNU-C4	-	NT	-	NT	+	-	+	+
16	SNU-C5	±	++	-	+++	+	+	+	-
17	Caco-2	-	++	++	+++	+	+	+	+
18	COLO201	-	++	-	+++	-	+	-	+
19	COLO205	-	-	-	++	+	+	+	+
20	COLO320	+++	NT	+++	NT	+	+	-	+
21	DLD-1	-	+++	-	+++	+	+	+	+
22	HCT-8	-	++	-	+++	+	-	+	-
23	HCT-15	-	++	-	+++	+	+	+	+
24	HCT-116	+++	NT	+++	NT	+	+	+	+
25	HT-29	+++	++	+++	+++	+	+	+	+
26	Lovo	+++	+++	+++	+++	+	+	+	+
27	LS174T	+++	NT	+++	NT	+	+	-	+
28	NCI-H716	+++	+++	+++	+++	+	+	-	+
29	SW403	+++	++	+++	+++	+	+	+	+
30	SW480	+++	NT	+++	NT	+	+	+	-
31	SW1116	-	NT	++	NT	+	+	-	+
32	WiDR	++	+++	+++	+++	+	+	-	+

5-aza: 5-aza-2’-deoxycytidine; NT: Not tested; M: The amplified product with primers recognizing methylated sequence; U: The amplified product with primers recognizing unmethylated sequence; +: Amplified product; -: Not amplified product.

Table 2 Summary of expressions, promoter methylation status and clinical associations of the MAGE-A1 and MAGE-A3 genes in 32 colorectal cancer cell lines and 87 colorectal cancer tissues

	% of expression			% of hypomethylation
	MAGE-A1		MAGE-A3	MAGE-A1		MAGE-A3
Cancer cell lines	59		66	81		81
Cancer tissues	NT1		NT	43		77
Normal tissues2	NT		NT	2		6
	MAGE-A1 hypomethylation			MAGE-A3 hypomethylation
	+	-	P	+	-	P
Location
Proximal	14 (31.1%)	31(68.9%)		34 (75.6%)	11 (24.4%)
Distal	17 (56.7%)	13(43.3%)	0.028	26 (86.7%)	4 (13.3%)	0.239
Sex
Male	17 (30.9%)	38(69.1%)		38 (69.1%)	17 (30.9%)
Female	20 (62.5%)	12(18.4%)	0.004	29 (90.6%)	3 (9.4%)	0.021

¹Not tested;

²Corresponding normal tissues of cancer tissues; +: Represent hypomethylation of MAGE genes; -: Represent no hypomethylation of MAGE genes.