Collision tumor of the rectum: A case report of metastatic gastric adenocarcinoma plus primary rectal adenocarcinoma

Table 1 Results of immunohistochemistry for primary rectal carcinoma component and both primary and metastatic carcinoma components of the stomach

Immunohisto-chemical markers	Antibody			Results
				Rectal tumor		Primary stomach
	Source	Clone	Dilution	Primary rectum	Metastatic stomach
CK7	DakoCytomation	OV-TL	1:200	Positive	Negative	Negative
CK20	DakoCytomation	Ks20.8	1:50	Positive	Positive	Positive
p53	DakoCytomation	DO-7	1:50	Positive	Positive	Positive
MUC2	Novocastra	Ccp58	1:500	Negative	Positive	Positive
MUC5AC	Novocastra	CLH2	1:500	Negative	Negative	Negative
CDX2	Novocastra	CDX2-88	1:100	Positive	Positive	Positive

Table 2 Results of loss of heteozygosity analysis for primary rectal carcinoma component and both primary and metastatic carcinoma components of the stomach

Microsatellitemarker	Chromoso-mal region	Tumorsuppressorgene	Loss of heterozygosity
			Rectal tumor		Primarystomach
			Primaryrectum	Metastaticstomach
D2S123	2p16	hMSH2	No (0.95)	No (0.93)	No (1.03)
D5S346	5q21	APC	No (1.31)	Yes (0.50)	Yes (0.54)
D17S250	17q11.2-12	P53	No (1.01)	Yes (0.41)	Yes (0.43)