Caffeic acid phenethyl ester modifies the Th1/Th2 balance in ileal mucosa after &gamma;-irradiation in the rat by modulating the cytokine pattern

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Aug 21, 2006 (publication date) through Sep 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 21, 2006; 12(31): 4996-5004
Published online Aug 21, 2006. doi: 10.3748/wjg.v12.i31.4996

Table 1 Sequences of custom primers for real-time quantitative PCR

Gene	Forward primer	Reverse primer
Th1 cytokines/receptors
IFN-γ	CACGCCGCGTCTTGGT	TCTAGGCTTTCAATGAGTGTGCC
IL-2	ATTTTCCAGGCACTGAAGATGTTT	CCCCATGATGCTCACGTTTA
IL-23	GCGTTCTCTTCTCCGTTCCA	TGCTCCGTGGGCAAAGAC
IL-12Rβ2	GGCTGCATCCTCCATTACAGA	CTGCTTATTGGATGTGAGTTTTG
Transcription factors
T-Bet	TCCTGTCTCCAGCCGTTTCT	CGCTCACTGCTCGGAACTCT
GATA-3	GGCGGCGAGATGGTACTG	TCTGCCCATTCATTTTATGGTAGA
Chemokine receptors
CXCR3	AGGTCAGTGAACGTCAAGTGCTAG	CAAAAAGAGGAGGCTGTAGAGGA
CCR5	CGGAACTTCTCCCCAACAAA	CTTTCTCTTCTGGACTCCCTACAACA
CCR4	GCCTCCAAGACAGACTTCCTTG	AGCGTTCGGTTCTAGTTTCCAC
Apoptotic Extrinsic factor/receptors
TNF-α	CATCTTCTCAAAATTCGAGTGACAA	TGGGAGTAGACAAGGTACAACCC
TNFR1	TGCCACGCAGGATTCTTTCTAAG	TGGGGGTTTGTGACATTTGC
TNFR2	CCCATCATTGAACCAAGCATCAC	AAGCAGGTCGCCAGTCCTAACATC
Fas	CGAATGCAAGGGACTGATAGC	TTCTGAGCAGTTGTTGTCGGTTT
FasL	AAGGCGGCCTTGTGATCA	TTGCAAGACTGACCCCGG
CTLA-4	GTGGAACTCATGTACCCACCG	GCATGGTTCTGGATCGATGAC
Apoptotic Intrinsic factors
XIAP	GGTGCAAGAAGCTATACGAATGG	AGTTGCTCCCAGATGTTTGGAG
Bax	GCTGACCTTGGAGCAGCC	ATGTTGTTGTCCAGTTCATCGC
Caspase-3	TGCGCCATGCTGAAACTG	CCTTCCGGTTAACACGAGTGA
Suppressors of Cytokine Signalling Proteins
SOCS1	ACACTCACTTCCGCACCTTC	AGCAGCTCGAAAAGGCAGTC
SOCS3	CCTCCAGCATCTTTGTC-GGAAGAC	TACTGGTCCAGGAACTCCCGAATG
Housekeeping
HPRT	GCTCGAGATGTCATGAAGGAGA	TCAGCGCTTTAATGTAATCCAGC

Citation: Grémy O, Benderitter M, Linard C. Caffeic acid phenethyl ester modifies the Th1/Th2 balance in ileal mucosa after γ-irradiation in the rat by modulating the cytokine pattern. World J Gastroenterol 2006; 12(31): 4996-5004
URL: https://www.wjgnet.com/1007-9327/full/v12/i31/4996.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i31.4996