Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 21, 2006; 12(31): 4996-5004
Published online Aug 21, 2006. doi: 10.3748/wjg.v12.i31.4996
Table 1 Sequences of custom primers for real-time quantitative PCR
GeneForward primerReverse primer
Th1 cytokines/receptors
IFN-γCACGCCGCGTCTTGGTTCTAGGCTTTCAATGAGTGTGCC
IL-2ATTTTCCAGGCACTGAAGATGTTTCCCCATGATGCTCACGTTTA
IL-23GCGTTCTCTTCTCCGTTCCATGCTCCGTGGGCAAAGAC
IL-12Rβ2GGCTGCATCCTCCATTACAGACTGCTTATTGGATGTGAGTTTTG
Transcription factors
T-BetTCCTGTCTCCAGCCGTTTCTCGCTCACTGCTCGGAACTCT
GATA-3GGCGGCGAGATGGTACTGTCTGCCCATTCATTTTATGGTAGA
Chemokine receptors
CXCR3AGGTCAGTGAACGTCAAGTGCTAGCAAAAAGAGGAGGCTGTAGAGGA
CCR5CGGAACTTCTCCCCAACAAACTTTCTCTTCTGGACTCCCTACAACA
CCR4GCCTCCAAGACAGACTTCCTTGAGCGTTCGGTTCTAGTTTCCAC
Apoptotic Extrinsic factor/receptors
TNF-αCATCTTCTCAAAATTCGAGTGACAATGGGAGTAGACAAGGTACAACCC
TNFR1TGCCACGCAGGATTCTTTCTAAGTGGGGGTTTGTGACATTTGC
TNFR2CCCATCATTGAACCAAGCATCACAAGCAGGTCGCCAGTCCTAACATC
FasCGAATGCAAGGGACTGATAGCTTCTGAGCAGTTGTTGTCGGTTT
FasLAAGGCGGCCTTGTGATCATTGCAAGACTGACCCCGG
CTLA-4GTGGAACTCATGTACCCACCGGCATGGTTCTGGATCGATGAC
Apoptotic Intrinsic factors
XIAPGGTGCAAGAAGCTATACGAATGGAGTTGCTCCCAGATGTTTGGAG
BaxGCTGACCTTGGAGCAGCCATGTTGTTGTCCAGTTCATCGC
Caspase-3TGCGCCATGCTGAAACTGCCTTCCGGTTAACACGAGTGA
Suppressors of Cytokine Signalling Proteins
SOCS1ACACTCACTTCCGCACCTTCAGCAGCTCGAAAAGGCAGTC
SOCS3CCTCCAGCATCTTTGTC-GGAAGACTACTGGTCCAGGAACTCCCGAATG
Housekeeping
HPRTGCTCGAGATGTCATGAAGGAGATCAGCGCTTTAATGTAATCCAGC