Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells

doi:10.3748/wjg.v12.i28.4478

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Jul 28, 2006 (publication date) through Apr 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Jul 28, 2006; 12(28): 4478-4484
Published online Jul 28, 2006. doi: 10.3748/wjg.v12.i28.4478

Table 1 Composition of hot water-extracted Lycium barbarum and Rehmannia glutinosa¹

Ingredient (mg/g)	Lycium barbarum extract	Rehmannia glutinosa extract
Carbohydrate	762.8	700.4
Protein	134.7	47.2
Fat	3.9	2.9
Moisture	221.6	92.4
Ash	0.5	0.4

¹The samples were at least triplicated.

Table 2 Effect of hot water-extracted Lycium barbarum (LBE) and Rehmannia glutinosa (RGE) on cell proliferation in rat hepatocellular carcinoma (H-4-II-E) cells (n = 6, mean ± SD)

	Cell proliferation (% of control)
	6 h	12 h	18 h	24 h
Control	100.0 ± 0.7^e^h^j	100.0 ± 8.6^e^h^j	100.0 ± 5.9^f^h^j	100.0 ± 5.9^f^h^j
1 g/L LBE	100.3 ± 9.8^f^h	100.7 ± 8.2^e^h	100.1 ± 5.7^f,h	97.8 ± 6.3^f^h
2 g/L LBE	84.4 ± 6.6^a^d^h	89.3 ± 7.5^a^c^h	83.8 ± 7.5^b^d^h	86.3 ± 2.9^b^d^h
5 g/L LBE	37.6 ± 6.9^b^d^f	23.8 ± 4.6^b^d^f	18.0 ± 5.3^b^d^f	15.1 ± 3.1^b^d^f
1 g/L RGE	99.5 ± 7.1^f^h^j	97.0 ± 13.4^h^j	111.4 ± 11.7^a^f^h^j	96.1 ± 5.3^f^h^j
2 g/L RGE	80.9 ± 18.4^b^d^h^j	88.3 ± 9.4^a^h^j	84.6 ± 14.4^b^d^h^j	84.4 ± 6.4^b^d^h^j
5 g/L RGE	58.3 ± 11.4^b^d^f^j	74.6 ± 7.0^b^d^f^j	57.1 ± 10.1^b^d^f^j	71.2 ± 6.7^b^d^f^j
10 g/L RGE	29.2 ± 6.9^b^d^f^h	29.5 ± 6.3^b^d^f^h	25.0 ± 5.7^b^d^f^h	24.2 ± 7.1^b^d^f^h

^aP < 0.05,

^bP < 0.01 vs control,

^cP < 0.05,

^dP < 0.01 vs 1 g/L corresponding treatment,

^eP < 0.05,

^fP < 0.01 vs 2 g/L corresponding treatment; ^gP < 0.05,

^hP < 0.01 vs 5 g/L corresponding treatment,

^jP < 0.01 vs 10 g/L corresponding treatment within the same column.

Table 3 Effect of hot water-extracted LBE and RGE on cell proliferation in human hepatocellular carcinoma (HA22T/VGH) cells after 24 h incubation (n = 6, mean ± SD)

	Cell proliferation (% of control)
Control	100.0 ± 4.2^d^f^h
2 g/L LBE	86.0 ± 5.9^b^c^h
5 g/L LBE	77.0 ± 5.1^a^b^h
10 g/L LBE	56.8 ± 3.9^b^d^f
2 g/L RGE	86.3 ± 5.9^b^f^h
5 g/L RGE	74.3 ± 7.5^b^d
10 g/L RGE	70.3 ± 7.7^b^d

^bP < 0.01 vs control;

^aP < 0.05,

^dP < 0.01 vs 2 g/L corresponding treatment;

^cP < 0.05,

^fP < 0.01 vs 5 g/L corresponding treatment;

^hP < 0.01 vs 10 g/L corresponding treatment.

Citation: Chao JCJ, Chiang SW, Wang CC, Tsai YH, Wu MS. Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells. World J Gastroenterol 2006; 12(28): 4478-4484
URL: https://www.wjgnet.com/1007-9327/full/v12/i28/4478.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i28.4478