Expression, purification and bioactivity of human augmenter of liver regeneration

Table 1 OD value of pEGFP-C1-hALR detected by ultraviolet spectrophotometer

Sample	abs	abs	bkgabs	260.0 nm	280.0 nm	Protein	Nucleic acid
ID	280.0 nm	260.60	320.0 nm	280.0 nm	260.0 nm	μg/mL	μg/mL
1	0.0319	0.0205	0.0057	1.7679	0.5657	3.1539	1.1124
	0.0337	0.0234	0.0086	1.6953	0.5899	3.9632	1.0440
2	0.0152	0.0073	-0.0009	1.9486	0.5132	0.6326	0.7177
	0.0159	0.0101	-0.0009	1.8485	0.5410	1.6853	0.8892

Table 2 Influence of different concentrations of hALR on DNA synthesis of primary rat hepatocyte CPM (mean ± SD)

CPM	1	2	3	4
	Without hALRcontrol group	5 ng/mL hALRmedium group	10 ng/mL hALRmedium group	20 ng/mL hALRmedium group
	n = 6	n = 6	n = 6	n = 6
1 1125 ± 158		P < 0.01	P < 0.01	P < 0.01
2 1802 ± 127			P < 0.01	P < 0.01
3 2764 ± 445				P < 0.01
4 3344 ± 417

Table 3 Influence of differentconcentrations of hALR on DNA synthesis of HepG₂ cell CPM ( mean ± SD)

CPM	1	2	3	4
	Without hALRcontrol group	5 ng/mL hALRmedium group	10 ng/mL hALRmedium group	20 ng/mL hALRmedium group
	n = 6	n = 6	n = 6	n = 6
1 1226 ± 158		P < 0.01	P < 0.01	P < 0.01
2 1802 ± 127			P < 0.01	P < 0.01
3 2764 ± 445				P < 0.01
4 3344 ± 417