Effects of inositol hexaphosphate on proliferation of HT-29 human colon carcinoma cell line

Table 1 P53 expression in IP₆-treated HT-29 cells (mean ± SD)

Concentration of IP6 (mmol/L)	Absorbance value
Control	0.6772 ± 0.0095
1.8	0.6161 ± 0.0203
3.3	0.5996 ± 0.0205
5.0	0.6067 ± 0.0130
8.0	0.5871 ± 0.0159
13.0	0.5817 ± 0.0158

Table 2 P21 expression in IP₆-treated HT-29 cells (mean ± SD)

Concentration of IP6 (mmol/L)	Absorbance value
Control	0.4868 ± 0.0486
1.8	0.6011 ± 0.0152
3.3	0.5138 ± 0.0336
5.0	0.6032 ± 0.0105
8.0	0.6078 ± 0.0066
13.0	0.5981 ± 0.0163

Table 3 PCNA expression in IP₆-treated HT-29 cells (mean ± SD)

Concentration of IP6 (mmol/L)	Absorbance value
Control	0.6407 ± 0.0096
1.8	0.6361 ± 0.0087
3.3	0.5904 ± 0.0302
5.0	0.4520 ± 0.0495
8.0	0.4788 ± 0.0357
13.0	0.5006 ± 0.0403

Table 4 Absorbance values assayed by MTT after continuous treatment with IP₆ (mean ± SD)

IP6 (mmol/L)	Time (d)
	0	2	4	6
Control	0.0847 ± 0.0021	0.1487 ± 0.0031	0.3083 ± 0.0240	0.3450 ± 0.0056
1.8	0.0857 ± 0.0045	0.1293 ± 0.0049	0.2320 ± 0.0155	0.3097 ± 0.0180
3.3	0.0837 ± 0.0042	0.1203 ± 0.0075	0.1770 ± 0.0227	0.2537 ± 0.0300
5.0	0.0873 ± 0.0057	0.1233 ± 0.0029	0.1547 ± 0.0042	0.2077 ± 0.0153
8.0	0.0857 ± 0.0021	0.0940 ± 0.0053	0.0873 ± 0.0032	0.0757 ± 0.0025
13.0	0.0847 ± 0.0032	0.0660 ± 0.0010	0.0530 ± 0.0026	0.0510 ± 0.0036

Table 5 Absorbance values assayed by MTT after IP₆ treatment for 6 h (mean ± SD)

IP6 (mmol/L)	Time (d)
	0	2	4	6
Control	0.0847 ± 0.0021	0.1487 ± 0.0031	0.3083 ± 0.0240	0.3450 ± 0.0056
1.8	0.0857 ± 0.0045	0.1397 ± 0.0023	0.2823 ± 0.0344	0.3303 ± 0.0080
3.3	0.0837 ± 0.0042	0.1333 ± 0.0015	0.2317 ± 0.0192	0.3383 ± 0.0025
5.0	0.0873 ± 0.0057	0.1277 ± 0.0025	0.2593 ± 0.0131	0.3240 ± 0.0082
8.0	0.0857 ± 0.0021	0.0940 ± 0.0053	0.0873 ± 0.0032	0.0757 ± 0.0025
13.0	0.0847 ± 0.0032	0.1157 ± 0.0035	0.2237 ± 0.0215	0.3020 ± 0.0080

Table 6 Absorbance values assayed by MTT after IP₆ treatment for 12 h (mean ± SD)

IP6 (mmol/L)	Time (d)
	0	2	4	6
Control	0.0847 ± 0.0021	0.1487 ± 0.0031	0.3083 ± 0.0240	0.3450 ± 0.0056
1.8	0.0857 ± 0.0045	0.1383 ± 0.0065	0.2660 ± 0.0056	0.3267 ± 0.0050
3.3	0.0837 ± 0.0042	0.1230 ± 0.0043	0.1990 ± 0.0554	0.3220 ± 0.0105
5.0	0.0873 ± 0.0057	0.1250 ± 0.0017	0.2290 ± 0.0350	0.3217 ± 0.0076
8.0	0.0857 ± 0.0021	0.1103 ± 0.0038	0.2693 ± 0.0711	0.2723 ± 0.0025
13.0	0.0847 ± 0.0032	0.0957 ± 0.0035	0.2363 ± 0.0299	0.2107 ± 0.0135

Table 7 Absorbance values assayed by MTT after IP₆ treatment for 24 h (mean ± SD)

IP6 (mmol/L)	Time (d)
	0	2	4	6
Control	0.0847 ± 0.0021	0.1487 ± 0.0031	0.3083 ± 0.0240	0.3450 ± 0.0056
1.8	0.0857 ± 0.0045	0.1323 ± 0.0040	0.2547 ± 0.0153	0.3330 ± 0.0122
3.3	0.0837 ± 0.0042	0.1277 ± 0.0099	0.2300 ± 0.0161	0.3123 ± 0.0115
5.0	0.0873 ± 0.0057	0.1317 ± 0.0032	0.2137 ± 0.0168	0.2990 ± 0.0190
8.0	0.0857 ± 0.0021	0.1067 ± 0.0021	0.1757 ± 0.0116	0.2093 ± 0.0105
13.0	0.0847 ± 0.0032	0.0807 ± 0.0021	0.0650 ± 0.0036	0.0857 ± 0.0051