Activation and dramatically increased cytolytic activity of tumor specific T lymphocytes after radio-frequency ablation in patients with hepatocellular carcinoma and colorectal liver metastases

Table 1 Stimulation indices (SI) of each patient before, 4 and 8 wk after RFA treatment

Tumor entity	CP	Etiology ofcirrhosis	SI before		SI 4 wk		SI 8 wk
			RFA		after RFA		after RFA
			CD4	CD8	CD4	CD8	CD4	CD8
HCC 1	A	alcohol	0.012	0.017	0.086	0.125	0.15	0.146
HCC 2	A	Hep C	0.021	0.019	0.16	0.138	0.176	0.136
HCC 3	B	Hep C	0.016	0.031	0.11	0.102	0.155	0.158
HCC 4	A	alcohol	0.015	0.019	0.07	0.11	0.079	0.112
HCC 5	B	alcohol	0.026	0.021	0.069	0.118	0.099	0.139
HCC 6	A	alcohol	0.02	0.02	0.16	0.125	0.146	0.134
Mean (SD)			0.018 (± 0.005)	0.021 (± 0.005)	0.109b (± 0.042)	0.120b (± 0.012)	0.134b (± 0.037)	0.138b (± 0.015)
Tumor entity	Primary		SI before		SI 4 wk		SI 8 wk
			RFA		after RFA		after RFA
			CD4	CD8	CD4	CD8	CD4	CD8
CRC metastasis 1	Sigma		0.025	0.023	0.123	0.158	0.129	0.155
CRC metastasis 2	Colon		0.035	0.029	0.155	0.176	0.199	0.143
CRC metastasis 3	Colon		0.01	0.017	0.069	0.11	0.126	0.125
CRC metastasis 4	Sigma		0.016	0.018	0.099	0.127	0.112	0.126
CRC metastasis 5	Rectum		0.011	0.02	0.103	0.183	0.175	0.176
CRC metastasis 6	Colon		0.02	0.021	0.14	0.147	0.149	0.137
Mean (SD)			0.019 (± 0.009)	0.021 (± 0.004)	0.115b (± 0.031)	0.150b (± 0.028)	0.148b (± 0.033)	0.144b (± 0.019)

CP: Child Pugh-Score of patients with liver cirrhosis; LC: liver cirrhosis; SI: Stimulation index, showing the ratio of IFNγ secretion of stimulated vs unstimulated T cells; HCC: hepatocellular carcinoma ; CRC metastasis: patients with colorectal cancer metastatic to the liver; Primary: location of the primary tumor; m.d.: missing data.

^bP < 0.0001 vs SI before RFA.

Table 2 Relative luminescence units (RLU) of cytosolic adenylate kinase released from the hepatoma cell line HepG2 for HCC patients and the colorectal carcinoma cell line CaCo for patients with colorectal carcinoma metastatic to the liver

Tumor entity	CP	Etiology of	AK release before	AK release 4 wk	AK release 8 wk
		cirrhosis	RFA	after RFA	after RFA
HCC 1	A	alcohol	4.8	398.46	535.87
HCC 2	A	Hep C	3.26	421.66	645.9
HCC 3	B	Hep C	4.88	578.63	845.99
HCC 4	A	alcohol	3.95	865.66	1025.21
HCC 5	B	alcohol	5	465.7	732.13
HCC 6	A	alcohol	4.66	751.33	924.66
Mean			4.42	580.24b	784.96b
(SD)			0.68	190.81	181.73
Tumor entity	Primary		AK releasebefore	AK release4 wk	AK release8 wk
			RFA	after RFA	after RFA
CRC metastasis 1	Sigma		2.13	568.32	816.55
CRC metastasis 2	Colon		3	947.47	1298.25
CRC metastasis 3	Colon		2.79	627.55	945.33
CRC metastasis 4	Sigma		2.56	699.45	845.54
CRC metastasis 5	Rectum		2.98	598.65	932.17
CRC metastasis 6	Colon		2.27	621.46	889.66
Mean			2.62	677.15b	954.58b
(SD)			0.37	139.38	175.42

Cells were co-incubated with PBMC isolated from each patient taken before, 4 and 8 wk after RFA. CP: Child Pugh-Score of patients with liver cirrhosis; LC: liver cirrhosis; HCC: hepatocellular carcinoma; CRC metastasis: patients with colorectal cancer metastastatic to the liver; Primary: location of the primary tumor; m.d.: missing data;

^bP < 0.0001 vs bioluminescence before RFA.