Hepatitis B virus x gene and cyanobacterial toxins promote aflatoxin B1-induced hepatotumorigenesis in mice

Table 1 Incidences of liver tumors caused by exposure to aflatoxin B₁ (AFB₁) and cyanobacterial toxins (CT) alone or in combination in HBV x (HBVx) gene transgenic mice and their wild-type littermates at 24 wk post-treatment

Group	Treatments			n	Adenoma (%)	Carcinoma (%)	Total (%)
	HBVx gene	AFB1	CT
1	-	-	-	40	0 (0)	0 (0)	0 (0)
2	+	-	-	10	0 (0)	0 (0)	0 (0)
3	-	+	-	20	1 (5.0)	0 (0)	1 (5.0)
4	+	+	-	11	1 (9.1)	0 (0)	1 (9.1)
	HBVx gene	AFB1	MC-LR
5	-	-	+	26	1 (3.9)	0 (0)	1 (3.9)
6	+	-	+	11	0 (0)	0 (0)	0 (0)
7	-	+	+	21	2 (9.5)	2 (9.5)	4 (19.0)a
8	+	+	+	15	2 (13.3)	1 (6.7)	3 (20.0)a
	HBVx gene	AFB1	NOD
9	-	-	+	26	0 (0)	0 (0)	0 (0)
10	+	-	+	11	0 (0)	0 (0)	0 (0)
11	-	+	+	9	2 (22.2)	1 (11.1)	3 (33.3)b,c
12	+	+	+	11	4 (36.4)	0 (0)	4 (36.4)d,e,f

MC-LR: Microcystin-LR; NOD: nodularin. ^aP < 0.05 vs group 1, ^bP < 0.01 vs group 1, ^cP < 0.05 vs group 9, ^dP < 0.01 vs group 1, ^eP < 0.05 vs group 3, ^fP < 0.01 vs group 9.