Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas

Table 1 Fraction of allelic loss (FAL) in tumors with different hypermethylation statuse

Promoterhypermethylation	FAL
Yes(++,+++)	0.098
Yes(+)	0.214
No	0.377	P = 0.03

FAL was estimated by allelic status at D16S3043, D16SS3050 and D16S3021. FAL (fraction of allelic loss) = number of loci showing LOH / number of informative loci in each tumor. The FALs of tumors with different hypermethylation status were calculated as the mean of FALs of individual tumors with the same hypermethylation status.

Table 2 Association between loss of heterozygosity and promoter hypermethylation of CDH1

Loss of heterozygosity
	Yes(%)	n(%)	Promoter hypermethylation
Yes(++,+++)	1(9.1)	17(34.7)
Yes(+)	2(18.2)	21(42.9)
No	8(72.7)	11(22.5)	P = 0.001

LOH status is defined by D16S3043