CD44v6 in peripheral blood and bone marrow as micro-metastasis of patients with gastric cancer

Table 1 Oligonucleotide primer and probe sequences used

Gene	Oligonucleotide	Location sequence PCR product	bp
CD44v	Upper primer	5′-TCCAGGCAACTCCTAGTAGT-3′	740
	Lower primer	5′-CAGCTGTCCCTGTTGTCGAA-3′
β-Actin	Upper primer	5’-CTACAATGAGCTGCGTGTGGC-3’	206
	Lower primer	5’-CAGGTCCAGACGCAGGATGGC-3’
Probe		5’-TGAGATTGGGTTGAAGAAATC-3’

Table 2 Relation between CD44v6mRNA expression in bone marrow and biologic behavior of gastric cancer

	Number of cases (n)		CD44v6mRNA/β-actin				P value
			-	+	+	+
Lymphnode metastasis	(+) Positive	38	3	2	4	29	0.0161
	(-) Negative	8	4	1	1	3
Clinicopathology	I-II	12	5	2	2	3
	III-IV	34	1	2	3	28	0.0007
Tissue type	D	32	1	2	4	25
	I	14	5	2	4	3	0.0003
Tumor size	>5 cm	24	3	2	6	13
	≤5 cm	22	3	3	4	12	0.4547

Table 3 Relation between CD44v6mRNA expression in bone marrow and biologic behavior of gastric cancer

	Number of cases (n)		CD44v6mRNA/β-actin				P value
			-	+	+	+
Lymphnode metastasis	(+) Positive	38	3	2	4	29	0.0131
	(-) Negative	8	4	1	1	2
Clinicopathology	I-II	12	5	2	2	3
	III-IV	34	2	2	3	27	0.0005
Tissue type	D	32	2	2	4	25
	I	14	5	2	4	3	0.0003
Tumor size	>5 cm	24	3	2	6	13
	≤5 cm	22	4	3	4	11	0.4240

Table 4 Quantitative expression of CD44v6mRNA in peripheral blood of patients with gastric cancer before and after surgery

Id	Sex	Yr	Path	Metastasis	>5cm	Histopathology	Real-time RT-PCR before surgery	(×102 copies/µg RNA) after surgery
1	M	56	D	+	T	III	3100	300
2	M	52	I	-	F	II	4.9	2.0
3	W	60	D	+	T	IV	580	66
4	W	69	D	+	T	III	696	72
5	W	41	D	+	T	IV	3200	460
6	M	39	D	+	T	IV	2650	244
7	M	61	I	-	F	II	26	9.0
8	W	42	D	+	T	IV	360	48
9	M	71	I	-	F	II	590	72
10	M	61	I	-	F	II	430	58
11	M	41	D	+	T	II	1230	142
12	W	47	D	+	T	IV	1670	186
13	M	32	D	+	T	IV	1340	166
14	M	49	I	+	T	III	280	36
15	W	61	D	+	T	II	1080	128
16	M	76	I	+	F	I	480	68
17	M	81	I	-	F	II	36	6.9
18	W	60	I	+	F	III	290	42
19	M	37	D	+	T	III	980	112
20	W	40	D	+	T	IV	1880	218
21	W	46	D	+	T	III	1896	122
22	W	68	I	-	T	IV	2208	342
23	M	72	I	-	T	III	1960	140
24	M	64	I	+	F	II	868	22
25	W	51	D	+	T	III	1020	100
26	M	32	D	+	T	IV	4100	289
27	W	26	D	+	T	IV	3908	432
28	M	72	I	-	F	II	680	107
29	W	74	I	-	T	III	1024	210
30	M	61	D	+	T	IV	1468	134
31	M	61	I		F	II	430	101
32	M	41	D	+	T	II	1230	11
33	W	47	D	+	T	III	1670	25
34	M	32	D	+	T	IV	1340	40
35	M	49	I	+	T	I	280	11
36	W	61	D	+	T	II	1080	120
37	M	76	I	+	F	I	480	91
38	M	81	I	-	F	I	36	137
39	W	60	I	+	F	II	290	43
40	M	37	D	+	T	III	980	69