Changes of the gastric endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma: An immunohistochemical quantitative study

Table 1 Antisera used in this study.

Antisera raised1	Code	Source	Dilution
CGA2	A430	DAKO Corp., Carpinteria	1:1000
Somatostatin	PUO421295	BioGenex Lab., San Ramon	1:20
Serotonin	BO68082C	BioGenex Lab., San Ramon	1:20
Gastrin	PUO190796	BioGenex Lab., San Ramon	1:20
CCK-82	750257	DiaSorin, Stillwater	0.388888889
Glucagon	927604	DiaSorin, Stillwater	1:2 000
HPP2	A610	DAKO Corp., Carpinteria	0.458333333

¹All antisera were raised in rabbits.

²CGA: chromogranin A, CCK-8: cholecystokinin-8, HPP: human pancreatic polypeptide.

Table 2 Regional distributions and frequencies of the endocrine cells in the stomach of the C57BL/6 mouse after implantation of 3LL (mean±SD).

Antisera	Non-implanted Sham		3LL-implanted group
	Fundus	Pylorus	Fundus	Pylorus
CGA1	17.30±2.16	41.206.16	5.20±2.20b	25.70±6.43b
Somatostatin	14.20±3.01	14.205.01	10.90±1.79b	6.20±1.75b
Serotonin	54.50±10.32	18.504.22	10.50±3.50b	7.90±2.88b
Gastrin	ND2	48.5011.10	ND	39.10±8.46a
CCK-82	ND	45.706.58	ND	46.90±6.92
Glucagon	3.10±0.74	ND	3.00±1.25	ND
HPP2	ND	ND	ND	ND

Number/1000 cells (n = 10);

¹CGA: chromogranin A, CCK-8: cholecystokinin-8, HPP: human pancreatic polypeptide;

²ND, not detected;

^aP<0.05 vs non-implanted sham by M-W test;

^bP<0.01 vs non-implanted sham by M-W test.