Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 14, 2005; 11(46): 7266-7271
Published online Dec 14, 2005. doi: 10.3748/wjg.v11.i46.7266
Table 1 Culture media used for the detection of bacterial flora in colonic biopsies
MediumTemperatureIncubation time (h)Organism
MacConkey's agar37 °C48-72Coliform bacilli (E coli and Klebsiella) and Proteus
Aerobic blood agar37 °C24-48Staphylococcus aureus
Anaerobic blood agar37 °C24-48Anaerobic bacteria as Bacteroides spp.
Microaerophilic Campy-blood agar42 °C24-48Campylobacter jejuni
MacConkey's selective medium25 °C24-48Yersinia Enterocolitica
Selenite broth enrichment medium and37 °C24-48Salmonella and Shigella
Salmonella-Shigella agar24
Table 2 Culture results of 60 colonic tissue specimens from 20 patients with lymphocytic colitis
OrganismPatients (n = 20)RS specimens (n = 20)SF specimens (n = 20)HF specimens (n = 20)
E coli121288
E coli + Proteus2200
Proteus1111
Klebsiella2222
Staphylococcus aureus1111
Total number18181212
Table 3 Histopathologic data of 60 colonic biopsies from 20 patients with lymphocytic colitis
FeatureRSSFHF
1 Surface epithelial damage
1+8108
2+666
3+646
2 Crypt distortion
1+171617
2+343
3+000
3 LP Inflammation
2+562
3+111012
4+446
4 Mean % of IEL232228
Table 4 Correlation between bacteria in tissue sections and histopathologic features in 60 colonic biopsies from 20 patients with lymphocytic colitis
FeatureBacteria
χ2P
+
1 Epithelial damage
1+1259.90.007
2+414
3+79
2 Crypt distortion
1+10400.4NS
2+28
3+00
3 LP Inflammation
2+2116.00.05
3+429
4+68