Effects of arginine supplementation on splenocyte cytokine mRNA expression in rats with gut-derived sepsis

Table 1 Composition of the semipurified diet (g/kg)

Ingredients	Arg-supplemented	Non-supplemented
Casein	180	220
Arg	20	-
Total nitrogen	35.2	35.2
Corn starch	677	677
Soybean oil	44	44
Vitamin1	10	10
Salt mixture2	35	35
Methyl-cellulose	30	30
Choline chloride	1	1
DL-methionine	3	3

¹The vitamin mix contained 0.6 mg/g thiamine hydrochloride, 0.6 mg/g riboflavin, 0.7 mg/g pyridoxine hydrochloride, 3 mg/g nicotinic acid, 1.6 mg/g calcium pantothenate, 0.02 mg/g D-biotin, 0.001 mg/g cyanocobalamin, 1.6 mg/g retinyl palmitate, 20 mg/g DL-a-tocopherol acetate, 0.25 mg/g cholecalciferol, and 0.005 mg/g menaquinone.

²The salt mixture contained 500 mg/g calcium phosphate diabasic, 74 mg/g sodium chloride, 52 mg/g potassium sulfate, 220 mg/g potassium citrate monohydrate, 24 mg/g magnesium oxide, 3.5 mg/g manganese carbonate, 6 mg/g ferric citrate, 1.6 mg/g zinc carbonate, 0.3 mg/g cupric carbonate, 0.01 mg/g potassium iodate, 0.01 mg/g sodium selenite, and 0.55 mg/g chromium potassium sulfate.

Table 2 Composition of the TPN solution (mL/L)

	Arg-supplemented	Non-supplemented
50% Glucose	412	400
20% Fat emulsion	50	50
10% Moriamin-SN1	450	556
Arg (g)	5	-
Infuvita2	8	8
3% NaCl	35	35
7% KCl	10	10
8.7%K3PO4	10	10
Ca-gluconate	10	10
MgSO4	4	4
ZnSO4	2	2
Choline chloride (g)	1	1

¹From Chinese Pharmaceuticals, Taipei, Taiwan. Contents per deciliter: 1 250 mg Leu, 560 mg Ile, 1 240 mg Lys acetate, 350 mg Met, 935 mg Phe, 650 mg Thr, 130 mg Trp, 450 mg Val, 620 mg Ala, 790 mg Arg, 380 mg Asp, 100 mg Cys, 650 mg Glu, 600 mg His, 330 mg Pro, 220 mg Ser, 35 mg Tyr, and 1 570 mg aminoacetic acid (Gly).

²From Yu-Liang Pharmaceuticals, Taoyuan, Taiwan. Contents per milliliter: 20 mg ascorbic acid, 660 IU vitamin A, 40 IU ergocalciferol, 0.6 mg thiamine HCl, 0.72 mg riboflavin, 8 mg niacinamide, 0.8 mg pyridoxine HCl, 3 mg D-panthenol, 2 mg dl-a-tocopheryl acetate, 12 µg biotin, 80 µg folic acid, and 1 µg cyanocobalamin.