Exogenous acid fibroblast growth factor inhibits ischemia-reperfusion-induced damage in intestinal epithelium via regulating P53 and P21WAF-1 expression

Table 1 Effect of aFGF on apoptotic rates in intestinal mucosa after ischemia-reperfusion insult (n = 6, mean±SD, %)

Groups	R group	A group
C group	19.67±3.50	19.67±3.50
I group	27.67±9.63	27.67±9.63
15 min after reperfusion	29.50±5.61	25.17±6.43
30 min after reperfusion	28.00±7.02	26.00±4.86
1 h after reperfusion	34.67±5.47a	29.83±7.08
2 h after reperfusion	50.67±6.95a	41.17±3.49ac
6 h after reperfusion	54.17±7.86a	42.83±5.23ac
12 h after reperfusion	64.33±6.47a	53.33±6.92ac
24 h after reperfusion	28.50±5.47	23.33±3.83
48 h after reperfusion	26.00±5.76	22.00±4.60

^aP<0.05 vs C group;

^cP<0.05 vs R group at matched time point.

Table 2 Effect of aFGF on protein expression of P53 and P21WAF-1 in intestinal mucosa after ischemia-reperfusion (n = 6, mean±SD, %)

Groups	P53		P21WAF-1
	R group	A group	R group	A group
C group	23.67±4.55	23.67±4.55	32.50±3.94	32.50±3.94
I group	29.17±4.45	29.17±4.45	35.83±4.83	35.83±4.83
15 min after reperfusion	27.00±3.90	23.83±5.08	34.67±4.76	31.50±4.37
30 min after reperfusion	30.67±3.98	26.67±3.78	36.17±3.31	37.00±4.86
1 h after reperfusion	33.17±3.19a	32.50±3.27a	39.83±4.58	37.83±4.36
2 h after reperfusion	40.33±3.50a	33.83±5.04ac	49.33±4.18a	41.83±6.65ac
6 h after reperfusion	50.50±4.23a	42.67±3.88ac	53.00±4.39a	44.67±4.46ac
12 h after reperfusion	45.67±5.65a	37.50±4.81ac	57.50±3.62a	49.33±3.78ac
24 h after reperfusion	30.00±6.13	25.33±3.33	34.50±6.02	30.00±3.79
48 h after reperfusion	28.17±8.89	25.67±4.32	33.83±7.31	30.67±4.18

^aP<0.05 vs C group;

^cP<0.05 vs R group at matched time point.

Table 3 Effect of aFGF on mRNA contents of P53 and P21WAF-1 genes in intestinal mucosa after ischemia-reperfusion (n = 6, mean±SD, %)

Groups	P53		P21WAF-1
	R group	A group	R group	A group
C group	23.5±6.0	22.4±2.5	26.7±2.0	24.6±4.1
I group	38.9±5.1a	34.8±4.2a	32.6±2.0a	31.7±2.0a
15 min after reperfusion	53.9±3.9a	45.8±10.7a	33.4±2.3a	24.0±3.4c
30 min after reperfusion	34.0±4.4a	41.9±5.2ac	23.3±4.2	22.9±2.0
1 h after reperfusion	48.5±3.2a	44.7±5.4a	24.8±2.3	23.8±3.2
2 h after reperfusion	46.7±5.4a	41.3±6.3a	26.5±2.7	24.9±3.0
6 h after reperfusion	48.8±5.2a	38.3±4.7ac	30.8±3.0a	20.9±2.6c
12 h after reperfusion	51.0±4.5a	34.1±4.8ac	32.7±1.6a	21.9±2.8c
24 h after reperfusion	55.3±7.3a	56.9±5.3a	28.8±3.1	28.5±5.0
48 h after reperfusion	56.4±8.3a	61.0±4.0a	29.0±2.3	28.9±3.8

^aP<0.05 vs C group;

^cP<0.05 vs R group at matched time point.