Review
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Nov 21, 2005; 11(43): 6735-6739
Published online Nov 21, 2005. doi: 10.3748/wjg.v11.i43.6735
Table 1 Detection of enzymatic inhibitory antibody to PDC by enzyme inhibition assay in non-PBC s
First authorYearCase studiedAntigen sourceSerum amount (μL)Serum dilutionPositivity rate (%)1
Teoh1991Normal subject, AIH, ALD, RA, SLECommercial PDC10020.3888888890 / 62 (<1.7)
Teoh1994Healthy subject, immunopathic diseasesCommercial PDC10020.3888888890 / 42 (<2.4)
Omagari1996Adult blood donors, healthy womenCommercial PDC2Undiluted0 / 186 (<0.6)
Schmit1999Healthy controls, viral hepatitis, AIHTRACE EMA kit4Undiluted0 / 92 (<1.1)
Jois2000Normal subject, AIC, AIH, ALD, RA, SLE, etc.Commercial PDC2Undilute4 / 1055 ( 0.4)
Hazama2000Healthy subject, ALD, viral hepatitis, fatty liverTRACE EMA kit4Undiluted0 / 50 (<2.0)
Jensen2000AIH, abnormal LFT patients, Normal blood donors, etc.TRACE EMA kit4Undiluted0 / 250 (<0.4)
Masuda2002Healthy subject, ALD, viral hepatitis, fatty liver, etc.TRACE EMA kit4Undiluted0 / 130 (<0.8)
Hazama2002Healthy subject, ALD, viral hepatitis, fatty liver, etc.TRACE EMA kit4Undiluted0 / 97 (<1.1)
Table 2 Interpretation of detection of anti-2-OADC by immunoblotting and enzyme inhibition assay
Immunoblotting (IgG/IgM/IgA)Enzyme inhibition assayInterpretationEstimated percentage (%)
PositivePositivePBC72 - 83
PositiveNegativeNon-anti-PDC positive PBC10 - 25
Immunoblotting false positive?10 - 15
NegativePositiveEnzyme inhibition assay false positive?Very rare
NegativeNegativeNon-PBCVery rare