Receptor-binding domain of SARS-Cov spike protein: Soluble expression in E.coli, purification and functional characterization

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Oct 21, 2005 (publication date) through Apr 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2005; 11(39): 6159-6164
Published online Oct 21, 2005. doi: 10.3748/wjg.v11.i39.6159

Table 1 Construction of recombinant plasmid and expression of engineering bacterium

Engineering bacterium	Recombinant plasmid	*Expression host (Escherichia colistrain)*	Expression Vector used	Fusion Tag
E.coli BL-TB	pET32-RBD	BL21(DE3)	pET32a	TrxA·tag S·tag
E.coli R-TB	pET32-RBD	Rosetta-gamiB(DE3)	pET32a	TrxA·tag S·tag
E.coli BL-GB	pGEX-RBD	BL21(DE3)	pGEX-4t-2	GST·tag
E.coli BL-MB	pET-MBP-RBD	BL21(DE3)	pET-MBP	MBP·tag

Citation: Chen J, Miao L, Li JM, Li YY, Zhu QY, Zhou CL, Fang HQ, Chen HP. Receptor-binding domain of SARS-Cov spike protein: Soluble expression in E.coli, purification and functional characterization. World J Gastroenterol 2005; 11(39): 6159-6164
URL: https://www.wjgnet.com/1007-9327/full/v11/i39/6159.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i39.6159