Anti-hepatoma effect of arsenic trioxide on experimental liver cancer induced by 2-acetamidofluorene in rats

Table 1 Changes of body and liver weights in SD rats (mean±SD)

Groups	Body weight	Body weight	Liver weight	Liver weight/body weight
A	272.25±35.51	74.25±20.71	12.475±1.9	3.49±0.40
B	247.56±63.94	58.44±16.99	13.11±3.15	4.42±0.60
C	253.00±28.63	58.25±24.01	12.54±1.132	4.08±0.50
D	258.22±49.99	40.44±40.15	11.89±1.59	4.24±1.00

Table 2 The number and distribution of tumor nodes on rat liver

Groups	n	2-4 mm diameter nodes	2-4 mm diameter nodes	Liver lobes with diffuse
A	8	0 (0)	1 (0.125)	0 (0)
B	9	50 (5.56)	9 (1)	17.5 (1.94)
C	8	137 (15.22)	13 (1.44)	29 (3.22)
D	9	223 (24.778)	49 (5.444)	39 (4.333)

Table 3 The percentages of cell cycle phases and PI

Groups	G0/G1 (%)	S (%)	G2/M (%)	PI (%)
A	93.31±3.01b	0.40±0.13bdf	6.28±3.26a	7.87±4.11bd
B	88.99±2.63	1.58±0.60b	9.43±2.29	17.14±6.08
C	87.34±4.75	2.05±0.62	10.61±4.33	20.77±5.28
D	84.26±2.53	3.01±0.51	12.73±2.40	24.46±6.49

^aP<0.05,

^bP<0.01, vs group D;

^dP<0.01, vs group C;

^fP<0.01, vs group B.

Table 4 The levels of serum enzymes

Groups	AST (μ/L)	ALT (μ/L)	TBi (μmol/L)	DBi (μmol/L)
A	61.46±9.46a	31.65±9.25	0.52±0.11	0.35±0.15
B	63.75±20.40a	34±12.58	0.62±0.27	0.40±0.24
C	61.18±13.00	33.45±16.44	0.51±0.14	0.30±0.12
D	108.98±29.86	40±16.12	0.47±0.13	0.60±0.28

^aP<0.05 vs group D. AST: aspartate aminotransferase, ALT: alanine aminotransferase, TBi: total bilirubin, DBi: direct bilirubin.

Table 5 Expression intensity of VEGF and count of MVD in rat liver tumor tissues

Groups	n	VEGF					MVD	Marks
		–	+	++	+++	++++
A	8	4	3	1	0	0	0.63±0.74a	15.75±3.99bd
B	9	1	5	2	1	0	1.33±0.87	28.22±9.28b
C	8	1	1	3	3	0	2.00±1.07	41.13±7.70
D	9	0	1	4	3	1	2.44±0.88	47.44±13.41

^aP<0.05,

^bP<0.01 vs group D;

^dP<0.01 vs group C.