Colchicine sensitizes human hepatocellular carcinoma cells to damages caused by radiation

Table 1 Colony assay of human HA22T/VGH hepatocellular carcinoma cells. Data are expressed as mean±SE

Colchicine (ng/mL)	0	1	2	4	8
RT 0 Gy	81.0 ± 4.0	78.7 ± 1.0	72.8 ± 3.3	57.5 ± 4.0	29.2 ± 2.7
1	78.3 ± 4.1	70.5 ± 5.1	52.7 ± 4.5	43.3 ± 4.3	22.3 ± 3.0
2	59.8 ± 3.5	53.5 ± 4.2	42.3 ± 3.6	33.2 ± 2.5	12.8 ± 3.0
4	51.2 ± 3.0	41.5 ± 3.2	32.8 ± 3.2	27.0 ± 3.3	11.5 ± 2.7
8	38.2 ± 5.6	27.3 ± 3.5	18.5 ± 1.8	15.3 ± 2.4	6.7 ± 1.8

Table 2 Cell cycle analysis of human HA22T/VGH hepatocellular carcinoma cells. Cell were treated with various doses of colchicine for 24 h and analyzed by flow cytometry

Colchicine (ng/mL)	Cell cycle distribution (%)
	G0/G1	S	G2+M
0	49.0 ± 1.4	24.6 ± 1.1	26.4 ± 1.0
1	51.4 ± 1.1	21.7 ± 1.4	26.9 ± 1.0
2	52.2 ± 1.5	21.5 ± 1.4	26.3 ± 0.9
4	51.6 ± 1.9	22.0 ± 1.5	26.5 ± 0.9
8	43.0 ± 2.1a	21.7 ± 1.7	35.4 ± 1.2b
16	17.6 ± 1.3b	23.5 ± 1.3	58.9 ± 2.2b

^aP < 0.05,

^bP < 0.01 vs colchicine treatment with 0 ng/mL.