Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1

Table 1 RT-PCR information: Primer sequences and PCR conditions.

Genes	Primer sequences (5-3)	Products bp	Annealing		Cycles
			°C	s
PDX-1	F, GTCCTGGAGGAGCCCAAC	360	58	60	30
	R, GCAGTCCTGCTCAGGCTC
Insulin	F, GCCTTTGTGAACCAACACCTG	261	62	60	30
	R, GTTGCAGTAGTTCTCCAGCTG
GLUT-2	F, TGCCACACTCACACAAGAC	260	54	60	30
	R, AGATTGTGGGCAGTTCATC
Glucokinase	F, CCCGAGGAGAACCACATT	208	56	60	30
	R, GGAACTCTGCCAGGATCT
IAPP	F, GCTGACATTGAAACATTA	360	56	60	30
	R, TATACAGGAAATCACTAGAA
Somatostatin	F, TGCGCTGTCCATCGTCCT	258	60	60	30
	R, GCCATAGCCGGGTTTGAGTT
E47	F, TCAGGCTGGCTTCCTGTCAG	224	62	60	30
	R, CCCTGCCGTATGCCTCACCT
NeuroD1	F, GCGTTAGCCTTCATGCGTCT	386	60	60	30
	R, GAGGCCCCAGGGTTATGAG
Isl-1	F, CGGCTTCAGCAAGAACGACT	290	58	60	30
	R, TCTTCTCCGGCTGCTTGTG
GAPDH	F,GTCAGTGGTGGACCTGACCT	415	55	60	30
	R,AGGGGAGATTCAGTGTGGTG

PCR conditions: denaturation at 94 °C for 30 s; annealing as listed in the table; extension at 72 °C for 1 min.