Inhibitory effect of tumor suppressor p33ING1b and its synergy with p53 gene in hepatocellular carcinoma

Table 1 G₀/G₁ cell cycle arrest analysis of hepatoma cells after cotransfection with p33^ING1b and wtp53 gene or control plasmids (mean±SD, %).

Cell line	Transfection plasmids	G0/G1 arrest
HepG2	p33ING1b	67.45±9.6a
	ap33ING1b	53.45±10.1
	p33ING1b+awtp53	55.61±8.3
	pcDNA3	58.78±10.3
PLC/PRF/5	p33ING1b	64.79±11.1
	p33ING1b+wtp53	78.16±10.5c
	ap33ING1b+ wtp53	60.17±9.8
	pcDNA3	56.56±8.8
Hep3B	p33ING1b	48.90±7.6
	p33ING1b+wtp53	55.91±10.1e
	ap33ING1b+ wtp53	50.92±8.5
	pcDNA3	52.87±7.0

^aP<0.05,

^cP<0.05, and

^eP>0.05 vs the control groups.

Table 2 Cloning efficiency of HepG2 cells after transfection of sense and antisense p33^ING1b gene (mean±SD).

Experiment	Number of colonies
	HepG2-p33ING1bb	HepG2-ap33ING1bd	HepG2-pcDNA3
1	65±7	149±12	86±9
2	68±15	151±10	93±11
3	72±10	148±11	94±10

^bP and

^dP<0.01, vs HepG2-pcDNA3 control.

Table 3 Relation of p33^ING1b and p53 protein expression in HCC tissues.

p33ING1b expression	Cases (n)	p53
		–	+	++	+++
HCC	57
–	33	19	7	3	4
+	10	4	3	2	1
++	10	0	0	3	7
+++	4	1	1	2	0
Para-cancerous tissues	51
–	38	38	0	0	0
+	13	13	0	0	0
++	0	0	0	0	0
+++	0	0	0	0	0
Normal liver tissues	12
–	10	10	0	0	0
+	2	2	0	0	0

Note: Staining was estimated as follows: –, negative; +, weak; ++, intermediate; +++, strong positive staining.