Effects of non-starch polysaccharides enzymes on pancreatic and small intestinal digestive enzyme activities in piglet fed diets containing high amounts of barley

Table 1 Formula and chemical composition of the basal diet

Ingredients (%)	Percentage
Barley	79.0
Soybean meal (dehulled, solvent)	11.0
Fishmeal	4.0
Yeast meal	2.0
Limestone	1.0
Dicalcium phosphate	1.2
Sodium chloride	0.3
L-Lysine-HCI (78%)	0.2
Vitamin-mineral premix1	1.3
Analyzed chemical composition (% as feed)
Digestible energy(Kcal/kg)2	2960
Crude protein	18.28
Crude fat	1.70
Crude fiber	5.26
Calcium	1.11
Phosphorus	0.48

¹The vitamin/mineral premix provided (per kg feed): 2 000 IU vitamin A, 200 IU vitamin D₃, 20 mg vitamin E, 1 mg vitamin K, l mg thiamine, 3 mg riboflavin, 10 mg d-pantothenic acid, 0.5 mg folic acid, 1 mg pyridoxine, 20 mg niacin, 10 μg cobalamin, 500 mg choline chloride, 0.1 mg biotin, 0.2 mg Se, 0.2 mg I, 80 mg Fe, 5 mg Cu, 2 mg Mn, and 80 mg Zn.

²Digest-ible energy was based on calculated values.

Table 2 Growth performance of piglets fed diets based on barley with and without NSP enzymes

	Dietary NSP enzymes level (%)
	0	0.15
Initial mass(kg)	13.91 ± 0.25	14.01 ± 0.17
Final mass(kg)	33.95 ± 0.38a	35.35 ± 0.40c
Average daily gain (g)	501.16 ± 16.18 a	532.34 ± 6.88c
Average daily feed intake (g)	1 224.00 ± 3.62a	1 250.15 ± 7.27c
Feed/Gain ratio	2.44 ± 0.03a	2.35 ± 0.02c

Values are presented as mean ± SD; n = 30 for average daily gain (ADG), n = 3 for average daily feed intake (ADFI) and feed/gain ratio per treatment. Means in a row with different letters differ significantly, P < 0.05.

Table 3 Effects of NSP enzymes on the digestive enzyme ac-tivities (U/g pancreas) in the pancreas of piglets

	Dietary NSP enzymes level (%)
	0	0.15
Protease	160.50 ± 17.49	188.86 ± 63.93
Chymotrypsin	1.09 ± 0.28	0.99 ± 0.19
Trypsin	32.14 ± 21.96	27.25 ± 6.79
Amylase	3 009.40 ± 157.19	2 957.02 ± 302.35
Lipase	89.08 ± 13.86	92.15 ± 13.86

Values are presented as mean ± SD; n = 3 per treatment.

Table 4 Effects of NSP enzymes on the digestive enzyme activities (U/mg protein) in the small intestinal contents of piglets

	Dietary NSP enzymes level (%)
	0	0.15
Protease	60.04 ± 12.86a	25.48 ± 4.98
Trypsin	37.21 ± 11.47a	8.90 ± 3.72c
Amylase	3600.45 ± 155.68a	1115.16 ± 93.32c
Lipase	68.68 ± 11.93a	41.06 ± 6.81c

Values are presented as mean ± SD; n = 3 per treatment. Means in a row with different letters differ significantly, P < 0.05.

Table 5 Effects of NSP enzymes on the digestive enzyme ac-tivities (U/mg) in jejunal mucosa of piglets

	Dietary NSP enzymes level (%)
	0	0.15
Maltase	28.49±6.45	29.89±10.017
Sucrase	8.13±0.62	8.92±2.16
Lactase	1.22±0.75	1.72±0.97
γ-Glutamyl transpeptidase	0.16±0.05	0.35±0.13a

Values are presented as mean ± SD; n = 3 per treatment. Means in a row with different letters differ significantly, P < 0.05.