Vascular endothelial growth factor antisense oligodeoxynucleotides with lipiodol in arterial embolization of liver cancer in rats

Table 1 Concentration of VEGF in supernatant of Walker-256 cells (pg/ml)(mean ± SD)

Group	0.25 μmol/L	0.5 μmol/L	1.0 μmol/L	2.0 μmol/L
Antisense ODNs	84.2 ± 2.2	79.2 ± 2.6	74.4 ± 2.1	65.2 ± 2.0
Sense ODNs	89.2 ± 1.9	90.4 ± 2.6	88.2 ± 1.9	88.6 ± 2.5
Control	90.2 ± 1.9	90.8 ± 1.8	89.4 ± 2.1	90.4 ± 1.7
F values	7.877	23.132	49.906	133.636
P values	0.0211	0.0021	0.0001	0.0001

¹SNK-q test: The difference between sense ODNs and control groups was not statistically significant.

Table 2 Inhibitory effect of supernatants of Walker-256 cells cultured with ODNs on ECV-304 proliferation (OD) (mean ± SD)

Group	0.25 μmol/L	0.5 μmol/L	1.0 μmol/L	2.0 μmol/L
Antisense ODNs	0.339 ± 0.012	0.332 ± 0.006	0.327 ± 0.008	0.321 ± 0.019
Sense ODNs	0.345 ± 0.021	0.348 ± 0.014	0.336 ± 0.022	0.359 ± 0.014
Control	0.357 ± 0.010	0.359 ± 0.008	0.356 ± 0.005	0.360 ± 0.009
F values	2.477	5.573	12.931	7.296
P values	0.164	0.0431	0.0071	0.0251

¹SNK-q test: The difference between sense ODNs and control groups was not statistically significant.

Table 3 Volumes and growth rates of transplanted liver tu-mors (mean ± SD)

Group	Volume of pre-TAE(mm3)	Volume of post-TAE(mm3)	Growth rate (%)
Control	212.3 ± 117.5	823.3 ± 426.1	403.9 ± 69.4
LP	174.6 ± 106.5	286.0 ± 186.4	177.9 ± 64.9
LP+ODNs	173.9 ± 91.8	337.6 ± 98.1	140.1 ± 33.8
F values	0.432	14.319	60.019
P values	0.653	0.0001	0.000

¹SNK-q test: The difference between antisense LP and LP+ODNs groups was not statistically significant.

Table 4 Expression of VEGF and MVD by immunohistochemi-cal staining (n=10)

Group	MVD (mean ± SD)	VEGF
		+++	++	+	-	Positive rate (%)
Control	73.2 ± 20.4	3	2	2	3	70
LP	80.3 ± 18.5	3	4	2	1	90
LP+ODNs	53.1 ± 18.4	1	2	2	5	50
Test values	F = 5.440	H = 3.731
P values	0.0101	0.1545

¹SNK-q test: The difference between LP and control groups was not statistically significant.