Selection, proliferation and differentiation of bone marrow-derived liver stem cells with a culture system containing cholestatic serum in vitro

Table 1 Sequence of primers and length of fragments

Gene	Primer	Fragment (bp)
HNF-1α	S:5’-AGCTGCTCCTCCATCATCAGA-3’	138
	A:5’-TGTTCCAAGCATTAAGTTTTCTATTCTAA-3’
HNF-3β	S:5’-CCTACTCGTACATCTCGCTCATCA-3’	68
	A:5’-CGCTCAGCGTCAGCATCTT-3’
CK-18	S:5’-GCCCTGGACTCCAGCAACT-3’	70
	A:5’-ACTTTGCCATCCACGACCTT-3’
CK-19	S:5’-ACCATGCAGAACCTGAACGAT-3’	83
	A:5’-CACCTCCAGCTCGCCATTAG-3’
Albumin	S:5’-CTGGGAGTGTGCAGATATCAGAGT-3’	141
	A:5’-GAGAAGGTCACCAAGTGCTGTAGT-3’
AFP	S:5’-GTCCTTTCTTCCTCCTGGAGAT-3’	145
	A:5’-CTGTCACTGCTGATTTCTCTGG-3’
TTR	S:5’-CAGCAGTGGTGCTGTAGGAGTA-3’	152
	A:5’-GGGTAGAACTGGACACCAAATC-3’
CYP2b1	S:5’-ACTTTCCTGGTGCCCACA-3’	157
	A:5’-TCCTTCTCCATGCGCAGA-3’