A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons

Table 1 Restriction digestion patterns that could identify HBV genotypes

Genotypes	Pattern	No. of sequences	Fragments obtained with
			BsrI	StyI	DpnI	HpaII	EaeI
A	A1	12	300 285	585	85 204 296	585	100 485
	A2	10	300 285	585	289 296	585	100 485
	A3	1	585	585	85 204 296	585	100 485
	A4	1	585	585	289 296	585	100 485
B	B1	33	126 459	585	289 296	585	100 485
	B2	2	126 184 275	585	289 296	585	100 485
C	C1	95	585	253 332	585	585	100 485
	C2	2	585	585	585	585	100 485
D	D1	36	585	585	289 296	585	100 485
	D2	1	585	585	585	585	100 485
	D3	1	300 285	585	585	585	100 485
E	E1	4	300 285	585	289 296	504 81	100 485
	E2	1	126 174 285	585	289 296	504 81	100 485
F	F1	19	585	585	289 256 40	90 495	100 485
	F2	1	585	585	289 296	90 495	100 485
G	G1	8	300 285	585	289 296	585	585
H	H1	3	585	585	289 296	90 495	100 485