Effect of SNPs in protein kinase Cz gene on gene expression in the reporter gene detection system

Table 1 Sequence of ten 49-bp fragments containing each al-lele of 5 SNPs

Fragment name	Sequence
rs809912G-forward	5’ ggggtaccccagccatcctccacc c gcccattctccatcc 3’
rs809912G-reverse	3’ gtcggtaggaggtgg g cgggtaagaggtaggttctagaag 5’
rs809912A-forward	5’ ggggtaccccagccatcctccacc t gcccattctccatcc 3’
rs809912A-reverse	3’ ggggtaccccagccatcctccacc a gcccattctccatcc 5’
rs436045A-forward	5’ ggggtacccagcagtgcctgtcag a tttggtccaagcagt 3’
rs436045A-reverse	3’ tcgtcacggacagtc t aaaccaggttcgtcactctagaag 5’
rs436045G-forward	5’ ggggtacccagcagtgcctgtcag g tttggtccaagcagt 3’
rs436045G-reverse	3’ tcgtcacggacagtc c aaaccaggttcgtcactctagaag 5’
rs427811T-forward	5’ ggggtaccgctcagtgtcctcttt t gagaaggtataggtg 3’
rs427811T-reverse	3’ gagtcacaggagaaa a ctcttccatatccacatctagaag 5’
rs427811G-forward	5’ ggggtaccgctcagtgtcctcttt g gagaaggtacaggtg 3’
rs427811G-reverse	3’ gagtcacaggagaaa c ctcttccatgtccacatctagaag 5’
rs385039G-forward	5’ ggggtacctgtttacagaagctac g ttgtaacacctgctc 3’
rs385039G-reverse	3’ caaatgtcttcgatg c aacattgtggacgagatctagaag 5’
rs385039A-forward	5’ ggggtacctgtttacagaagctac a ttgtaacacctgctc 3’
rs385039A-reverse	3’ caaatgtcttcgatg t aacattgtggacgagatctagaag 5’
rs411021C-forward	5’ ggggtaccgggggttgcggtgagc c gagattgtgccactg 3’
rs411021C-reverse	3’ ccccaacgccactcg g ctctaacacggtgacctctagaag 5’
rs411021T-forward	5’ ggggtaccgggggttgcggtgagc t gagattgtgccactg 3’
rs411021T-reverse	3’ ccccaacgccactcg a ctctaacacggtgacctctagaag 5’

Table 2 Transcriptional regulatory activity of each construct of PKCZ in HepG2 cells

Construct	Relative luciferase activity	P
pGL3-promoter	0.3533 ± 0.040
pGL3-rs411021C	0.5167 ± 0.064
pGL3-rs411021T	0.5100 ± 0.102	0.928
pGL3-rs436045A	0.3433 ± 0.051
pGL3-rs436045G	0.3767 ± 0.023	0.363
pGL3-rs427811T	0.6233 ± 0.064a
pGL3-rs427811G	0.4433 ± 0.068	0.029
pGL3-rs385039A	0.3500 ±0.044
pGL3-rs385039G	0.3467 ±0.015	0.907
pGL3-rs809912A	0.1800 ± 0.017a
pGL3-rs809912G	0.3033 ± 0.042	0.009

^aP < 0.05 in comparison between construct and pGL3-promoter vector.