Structure prediction and activity analysis of human heme oxygenase-1 and its mutant

Table 1 Bond angle, dihedral angle and the distance between atoms of △hHO-1

		Prime simulation	Optimized simulation
Bond angle	C24-N-CA	119.12	121.79
	CA-C-N26	115.92	115.12
Distance	CB-FE	5.55	5.58
between atoms	CA-FE	6.28	6.26
Dihedral angle	ω	175.40	175.61
	ϕ	-40.78	-34.45
	ψ	-60.70	-62.89

Table 2 whHO-1 activities after different purification

	Supernatant of DH5α lysates	30%-60% (NH4)2SO4	Q-Sepharose fast flow
whHO-1 activity (U·mg-1·h-1)	0.5	1.8	15
Purification fold	1.0	3.6	30