Inhibition of Fas/FasL mRNA expression and TNF-α release in concanavalin A-induced liver injury in mice by bicyclol

Table 1 Protection of bicyclol against Con A induced liver injury in mice as determined by ALT(U/L)

Time	Con A alone	Con A+bicyclol	Con A+bicyclol
		200 mg/kg	100 mg/kg
12 h	161.5 ± 63.11	27.1 ± 10.8b	50.1 ± 15.9a
16 h	165.8 ± 8.76	10.6 ± 12.8b	30.7 ± 27.4b

Data are mean ± SD of 8 mice per group.

^aP < 0.05,

^bP < 0.01. vs Con A alone. Serum ALT levels were measured 12 h and 16 h after Con A injection, respectively.

Table 2 Effect of bicyclol on liver histology by evaluation the degree of liver injury 12 h after Con A injection

Group	Grade of liver injury
	0	1	2	3
Normal (n = 7)	7	0	0	0
Con A alone (n = 7)	0	0	0	7
ConA+bicyclol 200 mg/kg (n = 8)	3	2	1	2
ConA+bicyclol 100 mg/kg (n = 7)	1	2	3	1

Histological degree of liver injury is classified according to the area of coagulative necrosis in hepatic lobules as follows: grade 0, no coagulative necrosis; grade 1, coagulative necrosis area < 10%; grade 2, coagulative necrosis area between 10% and 25%; grade 3, coagulative necrosis area > 25%.