Transcriptional regulation of human α1(I) procollagen gene in dermal fibroblasts

Table 1 Summary of CAT expression driven by various lengths of the 5’flanking sequence from human α1(I) procollagen gene

Name of transfected constructions	Putative promoters’ length (bp)	Relative activities of reporter gene (CAT, mean ± SD)1
pCOLH10.1	-105 to 42	0.10 ± 0.02
pCOLH10.27	-268 to 42	0.97 ± 0.04
pCOLH10.5	-496 to 42	0.20 ± 0.05
pCOLH10.9	-829 to 42	0.36 ± 0.09
pCOLH11.5	-1448 to 42	0.73 ± 0.11
pCOLH12.5	-2483 to 42	1.0

¹The expression of normalized CAT in the cells transfected with pCOLH₁2.5 was set as 1, the relative expression level of nor-malized CAT in the cells transfected with other constructs is shown in Table 1 (mean ± SD of three independent experiments).

Table 2 Effect of cytokines on reporter gene activity in cells transfected with pCOLH₁2.5

Cytokines		Relative value of reporter gene activity1	P value (compared to control)
Control		1.00 ± 0.15
TNFα	10 μg/L	0.43 ± 0.17	< 0.05
IFNα	1 × 105 U/L	0.62 ± 0.15	< 0.05
IFNν	1 × 105 U/L	0.60 ± 0.16	< 0.05

¹The reporter gene activity of the cells transfected with pCOLH₁2.5 was set as 1, the relative expression level of nor-malized CAT in transfected cells treated with cytokines was expressed as mean ± SD of three independent experiments.