Liver Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. May 15, 2004; 10(10): 1402-1408
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1402
Table 1 Titer of anti-HLCVECs antibody in immunized animals
D21D28D35D42D49D56
HLCVEC1:10001:30001:9000-1:90001:270001:27000+
Table 2 Construction of HLCVECs libraries in λ-ZAP expression vector and the number of SEREX-identified antigens
PatientAge (years)Primary sizeScreening serumClones screenedPositive clonescDNA fragmentKnown genesUnknown genes
HCC562 × 106immunized6 × 1053618153
Table 3 Results of immunoscreening of HLCVECs cDNA expression library by immunized BALB/C sera screening
CloneSize (bp)GeneLocalizationIdentityAccession number
EC192100HS.Protein disulfide7q3599%NM 004911.2
isomerase related protein
EC24700HS.Glucose regulated protein15q1598%NM 0O5313.3
EC261000HS.Chemokine ligand 14q12-q1399%BC 011976
EC291600HS.Inner membrane protein2p11.299%NM 006839.1
EC301800HS.Hypothetical protein Loc28324111q12.399%XM 208579.1
EC311700HS.genomic DNA, clone11q98%AP 002340.3
EC35700HS.BMP-615q13-q1598%NM 013372.1
EC362200HS.Glyceral dehyde-3-12p13.3198%BC 004109
phosphate dehydrogenase
EC381900HS.Replication factor C512q24.299%NM 007370.2
EC392100HS.BAC, CloneRP11-159112099%AC 010974.9
EC40800HS.Sjogren syndrome antigen B2q31.198%BC 020818
EC422400HS.X-ray repair complementing12q2399%AY 034001.1
defective repair in Chinese
EC51400HS.S100 calcium-binding protein A61q21100%BC 009017
EC521500HS.Protein kinase C19p1.198%NM 002743.1
EC531700HS.Heat shock 70 ku protein45q31.399%XM 114482.2
EC591900HS.DVS27 related protein9p24.199%BC 04785.1
EC621500HS.NAC alpha mRNA12q299%AY 034001.1
EC631500HS.Hypothetical protein DJ1042k10.22213.1100%HS 1042k10