Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I

Figure 1 Primer and locked nucleic acid probe positions designed for the detection of three genotypes of polymorphisms in the rt269 codon, ‘L1’, ‘I’ and ‘L2’. Arrows indicate the primer positions. Underlines indicate the probe positions. The numbers designate the nucleotide position on the hepatitis B virus P gene sequence. Boldface bases denote the different bases. The box represents the codon and amino acid sequences of rtL269 variants. This single nucleotide difference is the basis of their discriminative identification by locked nucleic acid probes in this study. The amino acid sequence is shown as one-letter amino acid symbols.