Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer

Figure 6 Methyl-CpG binding domain protein 1 positively regulates aerobic glycolysis and reactive oxygen species production in pancreatic cancer cells. A and B: Real-time polymerase chain reaction and western blotting demonstrated that methyl-CpG binding domain protein 1 (MBD1) was silenced in PANC-1 and MIA PaCa-2 cells; C: Knockdown of MBD1 decreased extracellular acidification rate values, indicating that MBD1 was a positive regulator of glycolysis; D: Silencing MBD1 in PANC-1 and MIA PaCa-2 cells increased oxygen consumption rate values, suggesting that MBD1 negatively regulated mitochondrial respiration; E: Downregulation of MBD1 decreased reactive oxygen species generation in PANC-1 and MIA PaCa-2 cells. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001 vs NC or mock group. MBD1: Methyl-CpG binding domain protein 1; ECAR: Extracellular acidification rate; OCR: Oxygen consumption rate.