Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer

Figure 2 Zinc finger E-box binding homeobox-1 regulates reactive oxygen species generation and mitochondrial membrane potentials. A and B: Decreased zinc finger E-box binding homeobox-1 (ZEB1) expression in PANC-1 and MIA PaCa-2 cells decreased reactive oxygen species generation, as demonstrated by using ROS assay kit analysis; C and D: Downregulation of ZEB1 increased mitochondrial membrane potential in PANC-1 and MIA PaCa-2 cells, which were confirmed by membrane potential probe JC-1 measurements. ^aP < 0.05, and ^bP < 0.01 vs NC or mock group. Error bars indicate mean ± SD. ROS: Reactive oxygen species; ZEB1: Zinc finger E-box binding homeobox-1.