Microbiota modification by probiotic supplementation reduces colitis associated colon cancer in mice

Figure 4 Probiotic supplementation modulates inflammation in colorectal carcinogenesis associated with colitis. Representative images (A) and inflammatory index (B) of mouse colons from control and probiotic groups at day 60 after azoxymethane injection stained with haematoxylin and eosin. ^cP < 0.001 Mann-Whitney U test, data representative of two independent experiments presented as mean and standard deviation. C: Spleen weight in control group (n = 21) and probiotic (n = 25) mice. Mann-Whitney U test. Data from two independent experiments are presented as mean and standard deviation. D: Concentration of serum cytokines analysed by Bio-Plex Multiplex cytokine assay at day 60 after injection with azoxymethane. ^aP < 0.05; Mann-Whitney U test. Data from two independent experiments, presented as mean and standard deviation. E: Representative western blot images of two independent experiments showing colon lysates of control and probiotic group mice; p-IKKα/β, IKKα, TNF-α, IL10 and β-tubulin.