Copyright ©The Author(s) 2018.
World J Gastroenterol. May 7, 2018; 24(17): 1839-1858
Published online May 7, 2018. doi: 10.3748/wjg.v24.i17.1839
Figure 6
Figure 6 Preparation of genetically modified TCR-expressing T cells. TCRα and TCRβ genes are cloned and amplified with the use of PCR from TAA-specific T cells, which are isolated and expanded ex vivo. The obtained TCRα and TCRβ genes are cloned into an expression vector, which is used for transfection into T cells. The transfected cells are adoptively transferred after ex vivo expansion.TAA: Tumor-associated antigen.