Copyright ©The Author(s) 2017.
World J Gastroenterol. Sep 7, 2017; 23(33): 6016-6029
Published online Sep 7, 2017. doi: 10.3748/wjg.v23.i33.6016
Table 1 Factors that influence effectiveness of dextran sodium sulfate to induce colitis
DSSMolecular weight40-50 kDa for tissue penetration (larger molecule does not penetrate colonic tissue well and smaller molecule has poor distribution)
Dosage concentrationRanges from 1.5%-3% used most frequently (1% with mild symptoms and delayed onset)
Duration of therapyAcute: 5-10 d administration
Manufacturer/batchChronic: 4-5 repeating cycles of DSS and sterile water
Various manufacturers with differing potency
HostGenetically susceptible strain of animalCertain strains are more susceptible to DSS colitis than other strains.
Susceptible strains: C3H/HeJ, C57BL/6, BALB/C
EnvironmentHousing ConditionsGroup vs individual unit, frequency of cage changes alters coprophagy by host
Microbial StateGerm-free vs specific pathogen free vs wild type