Copyright ©The Author(s) 2015.
World J Gastroenterol. Aug 7, 2015; 21(29): 8787-8803
Published online Aug 7, 2015. doi: 10.3748/wjg.v21.i29.8787
Table 1 Advantages and disadvantages of few of the currently available next generation sequencing techniques[15,16]
Techniques used in next generation sequencingAccuracyAdvantagesDisadvantages
454 Pyrosequencing99.9%Less amount of sample, long read lengths, large number of samples can be easily readHomopolymer errors Expensive
Shot gun Sequencing98%Short reads in short timeAssembly process is computationally expensive
Illumina Sequencing (Sequencing by synthesis)98%Accurate, quicker, reliable and cheapExpensive
Pacific Bio Sequencing (single molecule real-time sequencing)99.9%Fast and provides long read lengthExpensive equipment
Ion Torrent Sequencing (Ion semiconductor)98%Fast and less expensive equipmentMultiple monomer errors
SOLiD (Sequencing by Ligation) Sequencing99.9%Less expensive when compared to other methodsSlow and difficult to sequence palindromes