Tumor necrosis factor-α mediates JNK activation response to intestinal ischemia-reperfusion injury

Figure 5 Tumor necrosis factor-α mediated ischemia-reperfusion-induced mucosal apoptosis via caspase activation in intestinal mucosa. Total protein was extracted from intestinal mucosa, and subjected to SDS-PAGE and Western blotting analysis. β-actin was used as the control for loading. The results were expressed as a ratio to β-actin densitometry units. A: Western blotting analysis; B: The ratio of cleaved caspase-9 and β-actin; C: The ratio of cleaved caspase-3 and β-actin. Values are mean ± SE. Six rats were tested in each group. ^aP < 0.05 vs sham-operation (SO) rats pretreated with vehicle (SO + vehicle); ^cP < 0.05 vs ischemia-reperfusion(I/R) rats pretreated with vehicle (ischemia-reperfusion + vehicle). PTX: Pentoxifylline; IFX: Infliximab.