Glutamine depletion induces murine neonatal melena with increased apoptosis of the intestinal epithelium

Table 2 Nutrient composition of mouse milk and artificial amino acid milk

	Mouse milk	Artificial amino acid milk	CAM1	GDM	GRM
Osmotic pressure (mOsm/kg)	300	1800	1161	ND	ND
Glutamine (g/L)	ND	24.23	12.12	0	36.35
Fat (%)	22	10	16	16	16

¹Emulsified fat was added to artificial amino acid milk at a ratio of 1:1 to yield complete amino acid milk (CAM), because the proteins in the milk used in this study were in the form of amino acids, resulting in a very high osmotic pressure with a low amount of fat. As a result, the amount of amino acid contained in the CAM is 50% of the initial amount. GDM: Glutamine-deleted milk; GRM: Glutamine-rich milk; ND: Not determined.