Up-regulation of PIK3CA promotes metastasis in gastric carcinoma

Figure 2 Western blotting analyses of Akt phosphorylation. A: Protein from the indicated tissues was Western blotting with anti-phospho-Akt (Ser473) and anti-Akt to analyze the effect on activation of phosphatidylinositol 3-kinase (PI3K)/Akt signaling. β-actin was used as a loading control; B: Relative expression level of p-Akt (Ser473) protein quantified by grey analysis; C: Relative expression level of total Akt protein quantified by grey analysis. p-AKT, phosphorylated Akt; Lane 1: Normal gastric mucosa; Lane 2: Primary gastric cancer; Lane 3: Lymph node metastasis in gastric cancer; Lane 4: Distant metastasis in gastric cancer. ^a,b,cSignificant difference was indicated with different lower-case letters (n = 3).