Stable knockdown of heparanase expression in gastric cancer cells in vitro

Figure 3 Stable knockdown of heparanase abolishes the adhesion, migration and invasiveness of gastric cancer cells in vitro. A: In the adhesion assay, SGC-7901 cells stably transfected with shRNA exhibited a markedly reduced ability into adhere to the precoated matrigel, when compared to parental cells and vector (mock) group. However, the cells stably transfected with scrambled shRNA had similar ability for adhesion as parental cells; B: In the wound healing assay, the cells stably transfected with heparanase-specific shRNA demonstrated an impaired migration capacity when compared to the parental cells and vector (mock) group; C: In transwell analysis, stable transfection of shRNA abolished the invasive capabilities of SGC-7901 cells, when compared to the parental cells and vector (mock) group. The symbol (^a) indicates a significant (P < 0.05) decrease compared to parental cells. Experiments were performed in triplicate with essentially identical results.