Rapid Communication
Copyright ©2008 The WJG Press and Baishideng.
World J Gastroenterol. Dec 14, 2008; 14(46): 7127-7132
Published online Dec 14, 2008. doi: 10.3748/wjg.14.7127
Figure 5
Figure 5 Assays for IFNγ secretion ex vivo. Splenic CD3+ T cells were isolated from naïve BALB/c mice with MACS. T cells were primed with SGC-7901 tumor cell lysate-pulsed BM-derived DCs as described in Materials and Methods. The primed T cells (effector cells) were mixied and incubated with SGC-7901 or B16 target cells. Supernatant from each well was collected for measuring IFNγ production with the mouse IFNγ ELISA kit. Statistical analysis used the paired Student’s t test. The results are representative of three independent experiments. Data are given as mean ± SE. bP < 0.01, SGC-7901 versus B16 or Non.